Product Info Summary
| SKU: | A00367 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Factor VIII F8 Antibody
SKU/Catalog Number
A00367
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Factor VIII F8 Antibody catalog # A00367. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Factor VIII F8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00367)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Factor VIII. AA range:2161-2210
Reactive Species
A00367 is reactive to F8 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
267.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00367 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using Factor VIII Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from HUVEC cells, using Factor VIII Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Specific Publications For Anti-Factor VIII F8 Antibody (A00367)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Factor VIII F8 Antibody
Question
Do you have a BSA free version of anti-Factor VIII antibody A00367 available?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Factor VIII antibody A00367 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-06
Question
I am interested in to test anti-Factor VIII antibody A00367 on mouse brain for research purposes, then I may be interested in using anti-Factor VIII antibody A00367 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The products we sell, including anti-Factor VIII antibody A00367, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-10
Question
I was wanting to use your anti-Factor VIII antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
It shows on the product datasheet, A00367 anti-Factor VIII antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-03
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Factor VIII antibody A00367. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-25
Question
Would anti-Factor VIII antibody A00367 work for WB with brain?
K. Taylor
Verified customer
Asked: 2014-04-30
Answer
According to the expression profile of brain, F8 is highly expressed in brain. So, it is likely that anti-Factor VIII antibody A00367 will work for WB with brain.
Boster Scientific Support
Answered: 2014-04-30

