Anti-Factor VIII F8 Antibody

Product Name

Anti-Factor VIII F8 Antibody

View all F8 Antibodies

SKU/Catalog Number

A00367-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Factor VIII F8 Antibody catalog # A00367-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Factor VIII F8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00367-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Factor VIII

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00367-1 is reactive to F8 in Human, Mouse

Applications

A00367-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

267.009kDa

Background of F8

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

David A. Jans, J. Biol. Chem., Nov 1996; 271: 30781.
Eugene Estella, Diabetes, Aug 2006; 55: 2212 - 2219.
Yong Gu, J. Biol. Chem., May 1996; 271: 10816.
Jiuru Sun, J. Biol. Chem., Nov 1996; 271: 27802.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-Factor VIII F8 Antibody A00367-1

Western Blot (WB) analysis of specific cells using Factor VIII polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For F8 (Source: Uniprot.org, NCBI)

Alternative Names

AHF; Antihemophilic factor; coagulation factor VIII, procoagulant component; coagulation factor VIIIc; DXS1253E; F8Ccoagulation factor VIII; factor VIII F8B; FVIII; HEMAF8B; Procoagulant component F8 AHF, DXS1253EB, F8C, FVIII, HEMA, F8 coagulation factor VIII coagulation factor VIII|antihemophilic factor|coagulation factor VIII A1 domain|coagulation factor VIII C2 domain|coagulation factor VIII, procoagulant component|coagulation factor VIIIc|factor VIII F8B

For more info on F8, check out the F8 Infographic

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In this infographic, you will see the following information for F8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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