Anti-FAS-associated factor 1 FAF1 Antibody
Rabbit IgG polyclonal antibody for FAS-associated factor 1(FAF1) detection. Tested with WB in Human;Mouse;Rat.
Anti-FAS-associated factor 1 FAF1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
Threonyl-tRNA Synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that PL-7 antibodies (similar to Jo-1 antibodies) indicate an increased risk for lung involvement, but this needs to be confirmed for a larger number of cases.
|Product Name||Anti-FAS-associated factor 1 FAF1 Antibody
See all FAF1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for FAF1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. FAF1 information: Molecular Weight: 73954 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes.|
|Cite This Product||Anti-FAS-associated factor 1 FAF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1337)|
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|Anti-FAS-associated factor 1 FAF1 Antibody may replace the following items: sc 1885|sc 292983|sc 1886|sc 101255|sc 393965.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human FAF1(1-22aa MASNMDREMILADFQACTGIEN), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For FAF1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||11124|
|Species Of This Entry||Human|
|Protein Name||FAS-associated factor 1|
|Alternative Names||FAF1|CGI-03; Fas (TNFRSF6) associated factor 1; HFAF1s; TNFRSF6-associated factor 1; UBX domain protein 3A; UBX domain-containing protein 12; UBX domain-containing protein 3A; UBXD12FLJ37524; UBXN3AhFAF1FAS-associated factor 1|
|Post Tranlational Modifications||Ubiquitination; Phosphorylation; Cleavage; Oxidation|
|Gene Location||1p32.3, on Chromasome 1, gene sequence: NC_000001.11 (50437028..50960267, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For FAF1 (Source: Uniprot.org, NCBI)
|Protein Function||Ubiquitin-binding protein (PubMed:19722279). Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation (PubMed:26842564). Potentiates but cannot initiate FAS-induced apoptosis (By similarity).|
|Tissue Specificity||Most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes.|
|Research Areas||Apoptosis; Cancer|
|Related Diseases||Neoplasms; Malignant Neoplasms; Leukemia; Carcinoma; Mesothelioma; Myeloid Leukemia; Inflammation; Uniparental Disomy; Aortic Aneurysm, Abdominal; Leukemia, T-cell; Parkinson Disease; Leukemia, Myelocytic, Acute; Adult T-cell Lymphoma/leukemia; Cell Transformation, Neoplastic; Autoimmune Reaction; Stomach Neoplasms; Jaw Abnormalities; Non-neoplastic Disorder; Malignant Neoplasm Of Breast; Malignant Tumor Of Colon|
|Related Pathways||Cell Death; Pathogenesis; Cell Cycle; Oncogenesis; Localization; Rna Interference; Transport; Cell Adhesion; Cell Activation; Cell Migration; Osteoblast Differentiation; Proteolysis; Immune Response; Translation; Axon Guidance; Innate Immune Response; Cell Differentiation; Mitochondrial Translational Initiation; Nuclear Import; Translational Initiation|
|Background||Fas-associated factor-1(FAF1) is a Fas-binding pro-apoptotic protein that is a component of the death-inducing signaling complex in Fas-mediated apoptosis. FAF1 is involved in negative regulation of NF-kappaB activation. In addition to the previously known function as a component of the Fas death-inducing signaling complex, i.e. NF-kappaB activity suppressor by cytoplasmic retention of NF-kappaB p65 via physical interaction. hFAF1 was expressed abundantly in testis, skeletal muscle, and heart as 2.8 kb mRNA. Polyclonal antibody against hFAF1 detected 74 kD protein, a deduced protein size from the ORF and 40 kD protein in some cell lines.|
|Scientific References||PMID: 10462485 by Ryu S.-W., et al. Identification and characterization of human Fas associated factor 1, hFAF1.|
PMID: 21978668 by Pinheiro A.S., et al. The NLRP12 pyrin domain: structure, dynamics, and functional insights.
Our Boster Quality Guarantee for Anti-FAS-associated factor 1 FAF1 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Testis Tissue Lysate
Lane 2: MM453 Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: SW620 Cell Lysate
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