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Product Info Summary
| SKU: | M00151 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FAK PTK2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00151
BM4303 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FAK PTK2 Rabbit Monoclonal Antibody catalog # M00151. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FAK PTK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00151)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DDD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FAK
Reactive Species
M00151 is reactive to PTK2 in Human, Mouse, Rat
Observed Molecular Weight
119 kDa
Calculated molecular weight
119.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00151 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Recommend Dilution
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:50
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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Western blot analysis of FAK using anti-FAK antibody (M00151).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAK antigen affinity purified monoclonal antibody (Catalog # M00151) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAK at approximately 119 kDa. The expected band size for FAK is at 119 kDa.
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Immunohistochemical analysis of paraffin-embedded mouse spleen, using FAK Antibody.
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Immunofluorescent analysis of Hela cells, using FAK Antibody.
Specific Publications For Anti-FAK PTK2 Rabbit Monoclonal Antibody (M00151)
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Customer Q&As
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8 Customer Q&As for Anti-FAK PTK2 Rabbit Monoclonal Antibody
Question
We purchased anti-FAK Rabbit Monoclonal antibody for Flow Cytometry on corpus callosum in the past. I am using human, and We are going to use the antibody for ICC next. I was wanting to use examining corpus callosum as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
I looked at the website and datasheets of our anti-FAK Rabbit Monoclonal antibody and it appears that M00151 has been validated on human in both Flow Cytometry and ICC. Thus M00151 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-22
Question
We want using your anti-FAK Rabbit Monoclonal antibody for positive regulation of phosphatidylinositol 3-kinase activity studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
K. Wu
Verified customer
Asked: 2020-01-16
Answer
I appreciate your inquiry. This M00151 anti-FAK Rabbit Monoclonal antibody is tested on hela cells. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-16
Question
Our team were happy with the WB result of your anti-FAK Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma erythroleukemia focal adhesion. cell using this antibody. Is that expected? Could you tell me where is PTK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
From what I have seen in literature, cervix carcinoma erythroleukemia does express PTK2. Generally PTK2 expresses in cell junction, focal adhesion. cell. Regarding which tissues have PTK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8422239, 23503467
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Melanocyte, Pubmed ID: 8247543, 9422762
Placenta, Pubmed ID: 15489334
T-cell, Pubmed ID: 7692878
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-12-26
Question
Is M00151 specific for phosphorylated FAK "Active FAK" or can it recognize only total FAK?
Verified customer
Asked: 2019-12-04
Answer
The Anti-FAK PTK2 Rabbit Monoclonal Antibody (M00151) can only recognize total FAK.
Boster Scientific Support
Answered: 2019-12-04
Question
What is the sequence of the peptide immunogen for M00151?
Verified customer
Asked: 2019-12-03
Answer
The immunogen sequence of the Anti-FAK PTK2 Rabbit Monoclonal Antibody (M00151) is KPSRPGYPSPRSSEGFYPSP.
Boster Scientific Support
Answered: 2019-12-04
Question
Does M00151 contain any phosphorylation?
Verified customer
Asked: 2019-12-03
Answer
The Anti-FAK PTK2 Rabbit Monoclonal Antibody (M00151) doesn't contain any phosphorylation.
Boster Scientific Support
Answered: 2019-12-04
Question
We have observed staining in mouse cervix carcinoma. What should we do? Is anti-FAK Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
From literature cervix carcinoma does express PTK2. From Uniprot.org, PTK2 is expressed in corpus callosum, t-cell, brain, trachea, placenta, osteosarcoma, melanocyte, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have PTK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8422239, 23503467
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Melanocyte, Pubmed ID: 8247543, 9422762
Placenta, Pubmed ID: 15489334
T-cell, Pubmed ID: 7692878
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-24
Question
We are currently using anti-FAK Rabbit Monoclonal antibody M00151 for mouse tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-16
Answer
The anti-FAK Rabbit Monoclonal antibody (M00151) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-16
