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- Table of Contents
PTK2 antibody (M00151) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Immunofluorescent analysis of Hela cells, using FAK Antibody.
Designing a Western blot for PTK2? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PTK2 Western Blot GuideBundle Save
Anti-FAK PTK2 Rabbit Monoclonal Antibody
PTK2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
PTK2 antibody was positively detected by WB in:
human Hela whole cell, human MCF-7 whole cell, human HepG2 whole cell, human A549 whole cell, rat testis tissue, rat C6 whole cell, mouse testis tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PTK2 antibody was positively detected by IHC in:
mouse spleen tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PTK2 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Human
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-FAK PTK2 Rabbit Monoclonal Antibody
View all PTK2 Antibodies
View all PTK2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DDD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FAK
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
119 kDa
Calculated Molecular Weight
119.2 kDa
Cite This Product
Anti-FAK PTK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00151)
Protocols
Western Blot protocol for PTK2 antibody
Immunohistochemistry (IHC) protocol for PTK2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PTK2 antibody
Publications
Application:
Species:
Multi-omics integration and functional validation identify thrombospondin-1 as a functionally relevant mediator of doramectin-associated inhibition of glioblastoma migration and invasion
Published: 2026-08-25
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 8.70
Species: Human
PMID: 42641730
16α-Hydroxydehydrotrametenolic acid attenuates MDA-MB-231 triple-negative breast cancer xenograft growth and modulates integrin β1/c-Met signaling and glycolytic metabolism
Published: 2026-08-19
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 11.30
Species: Human
PMID: 42641546
Yijing Decoction improves premature ovarian failure in rats by activating VEGF/VEGFR-2/FAK pathway
Published: 2025-04-26
Journal: Journal of Ovarian Research
Application: IHC,WB
Impact Factor: 4.20
Species: Rat
PMID: 40287776
Transcription factor Runx2 changes the expression of some matricellular proteins in metastatic breast cancer cells
Published: 2022-04-19
Journal: MOLECULAR BIOLOGY REPORTS
Application: WB
Impact Factor: 2.74
Species: Human
PMID: 35441354
Effect of celecoxib on protein expression of FAK and Cx43 in DMBA induced rat tongue carcinoma cells
Published: 2019-11-01
Journal: European Review for Medical and Pharmacological Sciences
Application: IHC
Impact Factor: 2.72
Species: Rat
PMID: 31773683
Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo
Published: 2018-04-25
Journal: Acta Biomaterialia
Application: IF
Impact Factor: 6.38
Species: Rat,Human
PMID: 29702291
Research of shRNAmir inhibitory effects towards focal adhesion kinase expression in the treatment of gastric cancer
Published: 2014-11-21
Journal: Oncology Letters
Application: IHC
Impact Factor: 0.99
Species: Mouse
PMID: 25621028
Correlation between matrix metalloproteinase expression and activation of the focal adhesion kinase signaling pathway in herpes stromal keratitis
Published: 2013-11-13
Journal: Experimental and Therapeutic Medicine
Application: WB,IHC
Impact Factor: 0.34
Species: Human,Mouse
PMID: 24348806
Review
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Product Promise
We promise Anti-FAK PTK2 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00151
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 18, 2026