Product Info Summary
SKU: | M12622 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FAM50A Rabbit Monoclonal Antibody
SKU/Catalog Number
M12622
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-FAM50A Rabbit Monoclonal Antibody catalog # M12622. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FAM50A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12622)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
30F36
Isotype
IgG
Immunogen
A synthesized peptide derived from human FAM50A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M12622 is reactive to FAM50A in Human, Mouse, Rat
Applications
M12622 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
40.242kDa
Background of FAM50A
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FAM50A using anti-FAM50A antibody (M12622).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM50A antigen affinity purified monoclonal antibody (Catalog # M12622) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM50A at approximately 40 kDa. The expected band size for FAM50A is at 40 kDa.
Click image to see more details
Figure 2. IHC analysis of FAM50A using anti-FAM50A antibody (M12622).
FAM50A was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FAM50A Antibody (M12622) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of FAM50A using anti-FAM50A antibody (M12622).
FAM50A was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FAM50A Antibody (M12622) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of FAM50A using anti-FAM50A antibody (M12622).
FAM50A was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FAM50A Antibody (M12622) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For FAM50A (Source: Uniprot.org, NCBI)
Gene Name
FAM50A
Full Name
Protein FAM50A
Weight
40.242kDa
Superfamily
FAM50 family
Alternative Names
DXS9928EXAP-5 protein; family with sequence similarity 50, member A; HXC-26; Protein HXC-26; Protein XAP-5; XAP5HXC26,9F FAM50A 9F, DXS9928E, HXC-26, HXC26, MRXSA, XAP5 family with sequence similarity 50 member A protein FAM50A|protein XAP-5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FAM50A, check out the FAM50A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FAM50A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FAM50A Rabbit Monoclonal Antibody (M12622)
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No publications found for M12622
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