Anti-FAM65B/RIPOR2 Antibody Picoband™

Product Name

Anti-FAM65B/RIPOR2 Antibody Picoband™

View all RIPOR2 Antibodies

SKU/Catalog Number

A32101-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-FAM65B/RIPOR2 Antibody Picoband™ catalog # A32101-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-FAM65B/RIPOR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32101-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FAM65B/RIPOR2 recombinant protein (Position: L362-D1020).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A32101-1 is reactive to RIPOR2 in Human

Applications

A32101-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

118.519kDa

Background of RIPOR2

RHO family interacting cell polarization regulator 2 is a protein that in humans is encoded by the RIPOR2 gene. This gene encodes an atypical inhibitor of the small G protein RhoA. Inhibition of RhoA activity by the encoded protein mediates myoblast fusion and polarization of T cells and neutrophils. The encoded protein is a component of hair cell stereocilia that is essential for hearing. A splice site mutation in this gene results in hearing loss in human patients.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FAM65B/RIPOR2 using anti-FAM65B/RIPOR2 antibody (A32101-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM65B/RIPOR2 antigen affinity purified polyclonal antibody (Catalog # A32101-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM65B/RIPOR2 at approximately 120 kDa. The expected band size for FAM65B/RIPOR2 is at 119 kDa.

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Figure 2. Flow Cytometry analysis of HEL cells using anti-FAM65B/RIPOR2 antibody (A32101-1).
Overlay histogram showing HEL cells stained with A32101-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAM65B/RIPOR2 Antibody (A32101-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RIPOR2 (Source: Uniprot.org, NCBI)

Alternative Names

Rho family-interacting cell polarization regulator 2 RIPOR2 C6orf32, DFNB104, DIFF40, DIFF48, FAM65B, MYONAP, PL48 RHO family interacting cell polarization regulator 2 rho family-interacting cell polarization regulator 2|family with sequence similarity 65 member B|myogenesis-related and NCAM-associated protein homolog|protein FAM65B

For more info on RIPOR2, check out the RIPOR2 Infographic

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In this infographic, you will see the following information for RIPOR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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