Product Info Summary
| SKU: | A00925-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Pig |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-FAS-L FASLG Antibody
SKU/Catalog Number
A00925-1
Size
100ul
Form
Liquid
Description
FASLG (CD95L/TNFSF6) is a TNF-family ligand that binds FAS (CD95) to trigger extrinsic apoptosis and also engages NF-κB–linked non-apoptotic signaling in immune regulation contexts. Assay context: ELISA/IF for soluble vs cellular presentation; IHC for tissue distribution; Western blot for isoform/cleavage assessment. Often interpreted alongside cytokine activation markers such as IFNG and checkpoint molecules like PD-L1 (CD274) in immune-phenotyping studies.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FAS-L FASLG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00925-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human FAS ligand. AA range:101-150
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00925-1 is reactive to FASLG in Human, Mouse, Pig
Calculated molecular weight
31.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00925-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blotting validation for Anti-FAS-L FASLG Antibody A00925-1
Western blot (WB) analysis of FAS-L polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Immunohistochemistry validation of FASLG using Anti-FAS-L FASLG Antibody (A00925-1).
Immunohistochemical analysis of human-colon tissue. Anti-FAS-L at 1:200 (4°C
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Immunohistochemistry validation of FASLG using Anti-FAS-L FASLG Antibody (A00925-1).
Immunohistochemical analysis of human liver cancer tissue. Anti-FAS-L at 1:200 (4°C
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Immunohistochemistry validation of FASLG using Anti-FAS-L FASLG Antibody (A00925-1).
Immunohistochemical analysis of human stomach cancer tissue. Anti-FAS-L at 1:200 (4°C
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Immunohistochemistry validation of FASLG using Anti-FAS-L FASLG Antibody (A00925-1).
Immunohistochemical analysis of mouse colon tissue. Anti-FAS-L at 1:200 (4°CSpecific Publications For Anti-FAS-L FASLG Antibody (A00925-1)
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