Anti-Fascin-1 (Reed-Sternberg Cell Marker) Monoclonal Antibody

Product Name

Anti-Fascin-1 (Reed-Sternberg Cell Marker) Monoclonal Antibody

View all Fascin Antibodies

SKU/Catalog Number

M02147

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Fascin-1 (Reed-Sternberg Cell Marker) Monoclonal Antibody (Catalog # M02147). Tested in WB, Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Fascin-1 (Reed-Sternberg Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02147)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: FSCN1/418

Isotype

IgG2b, kappa

Immunogen

Full length recombinant human FSCN1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02147 is reactive to FSCN1 in Human, Rat

Applications

M02147 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

54.53kDa

Background of Fascin

Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Anti-Fascin-1 Mouse Monoclonal Antibody (FSCN1/418).

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Formalin-fixed, paraffin-embedded Rat Brain stained with Anti-Fascin-1 Mouse Monoclonal Antibody (FSCN1/418).

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Western Blot analysis of HeLa cell lysate using Anti-Fascin-1 Mouse Monoclonal Antibody (FSCN1/418).

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Flow Cytometric analysis of PFA-fixed K562 cells using Anti-Fascin-1 Mouse Monoclonal Antibody (FSCN1/418) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence analysis of HeLa cells labeling Fascin with Anti-Fascin-1 Mouse Monoclonal Antibody (FSCN1/418) -CF640 (Green). The nuclear counterstain is Hoescht (Blue). Membrane is Phalloidin 488 (Red).

Gene/Protein Information For FSCN1 (Source: Uniprot.org, NCBI)

Alternative Names

FAN1; FAN1,55 kDa actin-bundling protein; fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus); Fascin; FLJ38511; FSCN1; HSN; p55actin bundling protein; singed (Drosophila)-like (sea urchin fascin homolog like); singed-like (fascin homolog, sea urchin); Singed-like Protein; SNL; SNLfascin FSCN1 FAN1, HSN, SNL, p55 fascin actin-bundling protein 1 fascin|55 kDa actin-bundling protein|epididymis secretory sperm binding protein|fascin homolog 1, actin-bundling protein|singed-like (fascin homolog, sea urchin)

For more info on FSCN1, check out the FSCN1 Infographic

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In this infographic, you will see the following information for FSCN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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