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Product Info Summary
| SKU: | A07840-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Fascin 2/FSCN2 Antibody Picoband®
SKU/Catalog Number
A07840-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fascin 2/FSCN2 Antibody Picoband® catalog # A07840-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fascin 2/FSCN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07840-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Fascin 2/FSCN2 recombinant protein (Position: M1-K167).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07840-2 is reactive to FSCN2 in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
55.1 kDa
Background of FSCN2
Fascin-2 is a protein that in humans is encoded by the FSCN2 gene. It is mapped to 17q25.3. This gene encodes a member of the fascin protein family. Fascins crosslink actin into filamentous bundles within dynamic cell extensions. This family member is proposed to play a role in photoreceptor disk morphogenesis. A mutation in this gene results in one form of autosomal dominant retinitis pigmentosa and macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07840-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
"Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
"
Positive Control
WB: human placenta tissue, human U-87MG whole cell, human K562 whole cell, rat spleen tissue, rat ovary tissue, mouse lung tissue, mouse ovary tissue
Validation Images & Assay Conditions
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Western blot analysis of FSCN2 using anti-FSCN2 antibody (A07840-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: rat ovary tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse ovary tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSCN2 antigen affinity purified polyclonal antibody (Catalog # A07840-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSCN2 at approximately 60KD. The expected band size for FSCN2 is at 55KD.
Specific Publications For Anti-Fascin 2/FSCN2 Antibody Picoband® (A07840-2)
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1 Customer Q&As for Anti-Fascin 2/FSCN2 Antibody Picoband®
Question
We are currently using anti-Fascin 2/FSCN2 antibody A07840-2 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
F. Jones
Verified customer
Asked: 2015-09-16
Answer
The anti-Fascin 2/FSCN2 antibody (A07840-2) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-16
