Anti-F-box/LRR-repeat protein 3 FBXL3 Antibody

Product Name

Anti-F-box/LRR-repeat protein 3 FBXL3 Antibody

View all FBXL3 Antibodies

SKU/Catalog Number

A08330

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-F-box/LRR-repeat protein 3 FBXL3 Antibody (Catalog # A08330). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-F-box/LRR-repeat protein 3 FBXL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08330)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around aa.1~5 (M-K-R-G-G) derived from mouse FBXL3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08330 is reactive to FBXL3 in Mouse

Applications

A08330 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

48.707kDa

Background of FBXL3

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats and is localized in the nucleus. "Winston J.T.,et al.(1999) Curr. Biol. 9:1180-1182.
Carninci P. et al. (2005)Science 309:1559-1563.
The MGC Project Team, (2004)Genome Res. 14:2121-2127.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 49kd
Western blotting: 1:500~1:1000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Fbxl3 using anti-Fbxl3 antibody (A08330).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fbxl3 antigen affinity purified polyclonal antibody (Catalog # A08330) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Fbxl3.

Gene/Protein Information For FBXL3 (Source: Uniprot.org, NCBI)

Alternative Names

FBL3AF-box and leucine-rich repeat protein 3AF-box protein Fbl3a; FBL3F-box/LRR-repeat protein 3; F-box and leucine-rich repeat protein 3; F-box/LRR-repeat protein 3A; FBXL3A FBXL3 FBL3, FBL3AA, IDDSFAS, FBXL3 F-box and leucine rich repeat protein 3 F-box/LRR-repeat protein 3|F-box and leucine-rich repeat protein 3A|F-box protein Fbl3a|F-box/LRR-repeat protein 3A

For more info on FBXL3, check out the FBXL3 Infographic

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In this infographic, you will see the following information for FBXL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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