Anti-FE65/APBB1 Antibody

Product Name

Anti-FE65/APBB1 Antibody

View all FE65 Antibodies

SKU/Catalog Number

RP1059

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FE65/APBB1 Antibody catalog # RP1059. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FE65/APBB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1059)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FE65 recombinant protein (Position: Q295-A613). Human FE65 shares 95%and 96% amino acid (aa) sequence identity with mouse and rat FE65, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1059 is reactive to APBB1 in Human, Mouse

Applications

RP1059 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

77 kDa

Calculated molecular weight

77.244kDa

Background of FE65

APBB1 is also known as RIR or FE65. The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse

Validation Images & Assay Conditions

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Anti-FE65 antibody, RP1059, Western blotting
All lanes: Anti (RP1059) at 0.5ug/ml
WB: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 77KD
Observed bind size: 77KD

Gene/Protein Information For APBB1 (Source: Uniprot.org, NCBI)

Alternative Names

adaptor protein FE65a2; amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65); amyloid beta A4 precursor protein-binding, family B, member 1; Fe65; FE65amyloid beta A4 precursor protein-binding family B member 1; MGC:9072; Protein Fe65; RIR; stat-like protein APBB1 FE65, MGC:9072, RIR amyloid beta precursor protein binding family B member 1 amyloid-beta A4 precursor protein-binding family B member 1|adaptor protein FE65a2|amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)|stat-like protein

For more info on APBB1, check out the APBB1 Infographic

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In this infographic, you will see the following information for APBB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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