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Product Info Summary
| SKU: | PA1854 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FES Antibody
SKU/Catalog Number
PA1854
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FES Antibody catalog # PA1854. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FES Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1854)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FES.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1854 is reactive to FES in Human
Applications
PA1854 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
93 kDa
Calculated molecular weight
93.497kDa
Background of FES
FES (feline sarcoma oncogene) is an enzyme that in humans is encoded by the FES gene, also known as Proto-oncogene tyrosine-protein kinase Fes/Fps, Feline sarcoma/Fujinami avian sarcoma oncogene homolog, Proto-oncogene c-Fes, Proto-oncogene c-Fps, p93c-fes c-fes/fps protein, FPS, Oncogene FES, feline sarcoma virus, FPS. This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. Non-onc intervening sequences were present in the human counterpart. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia, but it is also involved in normal hematopoiesis. A truncated transcript has been identified that is generated utilizing a start site in one of the far downstream exons but a protein product associated with this transcript has not been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-FES antibody, PA1854, Western blotting
All lanes: Anti FES (PA1854) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 93KD
Observed bind size: 93KD
Protein Target Info & Infographic
Gene/Protein Information For FES (Source: Uniprot.org, NCBI)
Gene Name
FES
Full Name
Tyrosine-protein kinase Fes/Fps
Weight
93.497kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.10; EC 2.7.10.2; feline sarcoma oncogene; feline sarcoma virus; Fes; FPS; Proto-oncogene c-Fes; Proto-oncogene c-Fps; proto-oncogene tyrosine-protein kinase Fes/Fps; tyrosine-protein kinase Fes/Fps FES FPS FES proto-oncogene, tyrosine kinase tyrosine-protein kinase Fes/Fps|Oncogene FES, feline sarcoma virus|feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami avian sarcoma (PRCII) viral (v-fps) oncogene homolog|feline sarcoma oncogene|feline sarcoma/Fujinami avian sarcoma oncogene homolog|p93c-fes|proto-oncogene c-Fes|proto-oncogene c-Fps|proto-oncogene tyrosine-protein kinase Fes/Fps
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FES, check out the FES Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FES: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FES Antibody (PA1854)
Hello CJ!
No publications found for PA1854
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Anti-FES Antibody
Question
I see that the anti-FES antibody PA1854 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-11
Question
My lab would like to test anti-FES antibody PA1854 on human tongue for research purposes, then I may be interested in using anti-FES antibody PA1854 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The products we sell, including anti-FES antibody PA1854, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-19
Question
Will PA1854 anti-FES antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Dhar
Verified customer
Asked: 2017-01-25
Answer
You can see on the product datasheet, PA1854 anti-FES antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-25
Question
Would anti-FES antibody PA1854 work for WB with tongue?
S. Walker
Verified customer
Asked: 2014-08-05
Answer
According to the expression profile of tongue, FES is highly expressed in tongue. So, it is likely that anti-FES antibody PA1854 will work for WB with tongue.
Boster Scientific Support
Answered: 2014-08-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tongue using anti-FES antibody PA1854. Let me know if you need anything else.
A. Lewis
Verified customer
Asked: 2014-06-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-04
