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Product Info Summary
| SKU: | A10063 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-FEZ2 Antibody
SKU/Catalog Number
A10063
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FEZ2 Antibody (Catalog # A10063). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
FEZ2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-FEZ2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10063)
Host
Rabbit
Contents
FEZ2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FEZ2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human FEZ2. The immunogen is located within the last 50 amino acids of FEZ2.
Reactive Species
A10063 is reactive to FEZ2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
39.7 kDa
Background of FEZ2
Fasciculation and elongation protein zeta-2 (FEZ2) is a homolog to the mammalian FEZ1, itself an ortholog of the C. elegans UNC-76. In contrast to FEZ1, FEZ2 mRNA is widely expressed in mouse tissues. FEZ2 interacts with protein kinase C (PKC)-zeta substrate and induces neurite extension of PC12 cells when co-expressed with a constitutively active form of PKC-zeta, suggesting FEZ2 may play an important role in the morphological changes of various cells by associating with PKC-zeta in a tissue non-specific manner. FEZ2 can interact with FEZ1 through its c-terminal regions and especially its coiled-coil region. At least two isoforms of FEZ2 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10063 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FEZ2 antibody can be used for the detection of FEZ2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunoflourescence starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FEZ2 in mouse brain tissue lysate with FEZ2 antibody at (A) 0.5, and (B) 1 μg/mL.
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Immunofluorescence of human brain tissue using FEZ25 antibody at 5 μg/mL.
Specific Publications For Anti-FEZ2 Antibody (A10063)
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