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Product Info Summary
| SKU: | A07326 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-FGF-12 Antibody
SKU/Catalog Number
A07326
Size
100μl
Form
Liquid
Description
Boster Bio Anti-FGF-12 Antibody catalog # A07326. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF-12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07326)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human FGF12. AA range:31-80
Reactive Species
A07326 is reactive to FGF12 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
27.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07326 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of 22RV-1, BT474, SHSY5Y cells using FGF-12 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysate from 22RV-1 cells, using FGF12 Antibody.
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Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
Specific Publications For Anti-FGF-12 Antibody (A07326)
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Customer Q&As
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4 Customer Q&As for Anti-FGF-12 Antibody
Question
I am interested in to test anti-FGF-12 antibody A07326 on human retina for research purposes, then I may be interested in using anti-FGF-12 antibody A07326 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The products we sell, including anti-FGF-12 antibody A07326, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-08
Question
I see that the anti-FGF-12 antibody A07326 works with WB, what is the protocol used to produce the result images on the product page?
J. Zhang
Verified customer
Asked: 2018-01-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-01-30
Question
Is a blocking peptide available for product anti-FGF-12 antibody (A07326)?
A. Bhatt
Verified customer
Asked: 2015-05-20
Answer
We do provide the blocking peptide for product anti-FGF-12 antibody (A07326). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-05-20
Question
We are currently using anti-FGF-12 antibody A07326 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
D. Miller
Verified customer
Asked: 2013-08-16
Answer
The anti-FGF-12 antibody (A07326) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-16
