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- Table of Contents
FGF2 antibody (M00121) performs well in Flow Cytometry, IP, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of FGF2 using anti-FGF2 antibody (M00121).
FGF2 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-FGF2 Antibody (M00121) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
FGF2 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-FGF2 Antibody (M00121) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot for FGF2? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the FGF2 Western Blot GuideBundle Save
Anti-FGF2/Fgf Basic Rabbit Monoclonal Antibody
FGF2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IP, IHC, WB |
Tested Applications
FGF2 antibody was positively detected by WB in:
human Hela whole cell, human SIHA whole cell, human A549 whole cell, human U251 whole cell, human U2OS whole cell, human PC-3 whole cell, human SH-SY5Y whole cell, human A549- WT whole cell lysates, human A549-FGF2 KO whole cell lysates
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB
1:500-2000
IHC
1:50-200
IP
1:20
FC
1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-FGF2/Fgf Basic Rabbit Monoclonal Antibody
View all FGF2 Antibodies
View all FGF2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOHC-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FGF2
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
18,22,24 kDa
Calculated Molecular Weight
30.8 kDa
Cite This Product
Anti-FGF2/Fgf Basic Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00121)
Protocols
Western Blot protocol for FGF2 antibody
Publications
Application:
Species:
MicroRNA‑203‑3p inhibits the proliferation, invasion and migration of pancreatic cancer cells by downregulating fibroblast growth factor 2
Published: 2021-06-30
Journal: Oncology Letters
Application: WB
Impact Factor: 2.97
Species: Human
PMID: 34267818
Review
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Product Promise
We promise Anti-FGF2/Fgf Basic Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M00121
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 25, 2026