Anti-FGFR3 Rabbit Monoclonal Antibody

FGFR3 antibody

Boster Bio Anti-FGFR3 Rabbit Monoclonal Antibody catalog # M00200. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00200
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-FGFR3 Rabbit Monoclonal Antibody

View all FGFR3 Antibodies

SKU/Catalog Number

M00200

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-FGFR3 Rabbit Monoclonal Antibody catalog # M00200. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FGFR3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00200)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAID-6

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human FGFR3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00200 is reactive to FGFR3 in Human, Mouse, Rat

Applications

M00200 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

103 kDa

Calculated molecular weight

87.71kDa

Background of FGFR3

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For FGFR3 (Source: Uniprot.org, NCBI)

Gene Name

FGFR3

Full Name

Fibroblast growth factor receptor 3

Weight

87.71kDa

Superfamily

protein kinase superfamily

Alternative Names

CD333; CEK; EC 2.7.10; FGF R3; FGFR3; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism FGFR3 ACH, CD333, CEK2, HSFGFR3EX, JTK4 fibroblast growth factor receptor 3 fibroblast growth factor receptor 3|FGFR-3|fibroblast growth factor receptor 3 variant 4|fibroblast growth factor receptor 3-S|hydroxyaryl-protein kinase|tyrosine kinase JTK4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FGFR3, check out the FGFR3 Infographic

FGFR3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FGFR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-FGFR3 Rabbit Monoclonal Antibody

Question

Does M00200 anti-FGFR3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

As indicated on the product datasheet, M00200 anti-FGFR3 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-23

Question

I have a question about product M00200, anti-FGFR3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00200 anti-FGFR3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-03

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-FGFR3 Rabbit Monoclonal antibody M00200. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-04-30

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-30

Question

I am interested in to test anti-FGFR3 Rabbit Monoclonal antibody M00200 on mouse erythroleukemia for research purposes, then I may be interested in using anti-FGFR3 Rabbit Monoclonal antibody M00200 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-01-11

Answer

The products we sell, including anti-FGFR3 Rabbit Monoclonal antibody M00200, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-01-11

Question

Is a blocking peptide available for product anti-FGFR3 Rabbit Monoclonal antibody (M00200)?

S. Carter

Verified customer

Asked: 2016-12-21

Answer

We do provide the blocking peptide for product anti-FGFR3 Rabbit Monoclonal antibody (M00200). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-12-21

Question

Will anti-FGFR3 Rabbit Monoclonal antibody M00200 work for WB with erythroleukemia?

A. Kulkarni

Verified customer

Asked: 2014-04-04

Answer

According to the expression profile of erythroleukemia, FGFR3 is highly expressed in erythroleukemia. So, it is likely that anti-FGFR3 Rabbit Monoclonal antibody M00200 will work for WB with erythroleukemia.

Boster Scientific Support

Answered: 2014-04-04

Order DetailsPrice
M00200

100μL

$370
M00200-30ul

30μL sample

$99
M00200-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M00200
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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