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Product Info Summary
| SKU: | A01200 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-FHIT Antibody
SKU/Catalog Number
A01200
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FHIT Antibody (Catalog # A01200). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
FHIT antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-FHIT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01200)
Host
Rabbit
Contents
FHIT Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FHIT antibody was raised against an 18 amino acid peptide near the carboxy terminus of human FHIT. The immunogen is located within the last 50 amino acids of FHIT .
Cross-reactivity
FHIT antibody is human and mouse reactive.
Reactive Species
A01200 is reactive to FHIT in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
16.9 kDa
Background of FHIT
FHIT is member of the histidine triad gene family and is a diadenosine involved in purine metabolism (1). FHIT is also thought to be a tumor suppressor gene and is involved in multiple apoptotic pathways (1,2). The FHIT gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene (3). Aberrant transcripts from this gene have been found in multiple carcinomas (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01200 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FHIT antibody can be used for detection of FHIT by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 μg/ml.
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Immunofluorescence of FHIT in HeLa cells with FHIT antibody at 5 μg/mL.
Specific Publications For Anti-FHIT Antibody (A01200)
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