FHIT Antibodies

AND FHIT ELISA kits, FHIT Proteins

Many biological assays use antibodies to detect FHIT, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with FHIT in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop FHIT antibodies...

We validate the specificity of these antibodies to FHIT by testing them on tissues known to express FHIT positively and negatively. Browse below to find the FHIT antibody that suites your experiment. We have 12 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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FHIT Infographic by Boster Bio

All FHIT Products

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Applications

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Anti-FHIT Antibody Picoband®

1 Q&As

Western blot analysis of FHIT using anti-FHIT antibody (PB9181)

IHC analysis of FHIT using anti-FHIT antibody (PB9181)

Flow Cytometry analysis of U20S cells using anti-FHIT antibody (PB9181)

Human, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # PB9181
100 μg/vial

Anti-FHIT Antibody Picoband® (monoclonal, 26H7)

Western blot analysis of FHIT using anti-FHIT antibody (M01200)

IF analysis of FHIT using anti-FHIT antibody (M01200)

Flow Cytometry analysis of 293T cells using anti-FHIT antibody (M01200)

Human

Flow Cytometry, IF, ICC, WB

$370
Cat # M01200
100 μg/vial

FHIT Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from A549 cells

Human

ELISA

$480
Cat # EKC1215
1 kit, containing one 96-well plate and all necessary reagents

Anti-FHIT Antibody

Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 μg/ml

Immunofluorescence of FHIT in HeLa cells with FHIT antibody at 5 μg/mL

Human, Mouse

ELISA, IF, WB

$481
Cat # A01200
0.1 mg

Anti-FHIT (E116) Antibody

Western blot (WB) analysis of FHIT (E116) pAb at 1:500 dilution
Lane1:SK-OVCAR3 whole cell lysate(40ug)
Lane2:The Kidney tissue lysate of Mouse(40ug)
Lane3:MCF-7 whole cell lysate(40ug)
Lane4:SGC7901 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of FHIT (E116) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Immunoprecipitation of MCF-7 cell lysate using FHIT (E116) polyclonal antibody (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 )

Human, Mouse

IP, IF, IHC, WB

$405
Cat # A01200-1
100ul

Anti-FHIT Antibody

Western blot analysis of extracts of various cell lines, using FHIT antibody at 1:1000 dilution

Human, Mouse, Rat

$405
Cat # A01200-2
100ul

Anti-Phospho-Fhit (Y114) Antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using FHIT (Phospho-Tyr114) Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FHIT (Phospho-Tyr114) Antibody

Human, Mouse, Rat

ELISA, IF, IHC

$380
Cat # A01200Y114
100ul

FHIT (NM_001166243) Human Recombinant Protein

$1249
Cat # PROTP49789
20 µg

Anti-FHIT Antibody

Overlay histogram showing HepG2 cells stained with M01200-1 (green line)

Human

Flow Cytometry, WB

$299
Cat # M01200-1
80 µl

Anti-FHIT Mouse Monoclonal Antibody [Clone ID: OTI2C2]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHIT (Right lane) cDNA for 48 hrs and lysed

Western blot analysis of extracts (35ug) from 2 tissue lysates by using anti-FHIT monoclonal antibody (1:500)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FHIT mouse monoclonal antibody

Human, Mouse, Rat

IHC, WB

$523
Cat # M01200-2
100 µl

Anti-FHIT Rabbit Monoclonal Antibody

Western blot analysis of FHIT expression in Rat kidney lysate

Human, Rat

$370
Cat # M01200-3
100 μl/vial

FHIT (NM_002012) Human Over-expression Lysate

FHIT Human Over-expression Lysates NM_001166243

Human

$628
Cat # LS002272
20 µg, 100 µg

FHIT Overview

Facts about Bis(5'-adenosyl)-triphosphatase.

FHIT 3D structure. Source: alphafold

Bis(5'-adenosyl)-triphosphatase (FHIT)

Can also hydrolyze P(1)-P(4)-bis(5'- adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5.

Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis.

Gene Information

Human
Gene Name:	FHIT
Uniprot:	P49789
Entrez:	2272

Family

Belongs to:

No superfamily

Alternative Names

AP3A hydrolase; AP3AaseFRA3Bbis(5'-adenosyl)-triphosphatase; Diadenosine 5'-5'''-P1; dinucleosidetriphosphatase; EC 3.6.1.29; fragile histidine triad gene; Fragile histidine triad protein; P3-triphosphate hydrolase; tumor suppressor protein

Molecular Weight

Mass (kDA):
16.858 kDA

Genomic Context


Human

Location:	3p14.2
Sequence:	3; NC_000003.12 (59747277..61251461, complement)

Tissue Specificity

Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines.

Subcellular Localizations

Cytoplasm. Mitochondrion. Nucleus.

Diseases

Neoplasms
Malignant Neoplasms
Carcinoma
Carcinogenesis
Malignant Squamous Cell Neoplasm
Malignant Neoplasm Of Lung
Cell Transformation, Neoplastic
Lung Neoplasms
Adenocarcinoma
Neoplasm Metastasis
Malignant Paraganglionic Neoplasm
Non-small Cell Lung Carcinoma
Mammary Neoplasms

Malignant Neoplasm Of Breast
Malignant Tumor Of Cervix
Chromosome Fragility
Uterine Cervical Neoplasm
Stomach Neoplasms
Esophageal Neoplasms
Precancerous Conditions

Interacting Proteins

CTNNB1
LEF1

Pathways

Methylation
Pathogenesis
Cell Cycle
Cell Proliferation
Dna Methylation
Cell Growth
Reverse Transcription
Mismatch Repair
Dna Repair
Cell Death
Oncogenesis
Induction Of Apoptosis
Cell Differentiation

Localization
Translation
Gene Silencing
Chromosome Breakage
Dna Replication
Dna Hypermethylation
Metaphase

PTMs

Methylation
Phosphorylation
Cleavage
Demethylation

Reduction
Acetylation
Deamination
Deacetylation

Amidation

Research Areas

Apoptosis
Cancer
Cellular Markers
Lipid and Metabolism
Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/FHIT

References

PMID: 8598045 by Ohta M., et al. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers.

PMID: 9012482 by Druck T., et al. Structure and expression of the human FHIT gene in normal and tumor cells.

2 Publications of Boster FHIT Antibodies & ELISA Kits

PubMed ID: 3000101, Neuroprotective Properties of Picroside II in a Rat Model of Focal Cerebral Isch...
PubMed ID: 21151457, Li Q, Li Z, Xu Xy, Guo Yl, Du F. Int J Mol Sci. 2010 Nov 16;11(11):4580-90. Doi:...

*More publications can be found for each product on its corresponding product page