Anti-Fibroblast activation protein, alpha/FAP Antibody Picoband™

Product Name

Anti-Fibroblast activation protein, alpha/FAP Antibody Picoband™

View all Fibroblast Activation Protein alpha/FAP Antibodies

SKU/Catalog Number

A00422-5

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fibroblast activation protein, alpha/FAP Antibody Picoband™ catalog # A00422-5. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Fibroblast activation protein, alpha/FAP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00422-5)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Fibroblast activation protein, alpha/FAP recombinant protein (Position: Q65-Q580).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00422-5 is reactive to FAP in Human

Applications

A00422-5 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

97 kDa

Calculated molecular weight

87.713kDa

Background of Fibroblast Activation Protein alpha/FAP

Fibroblast activation protein alpha (FAP-alpha) also known as prolyl endopeptidase FAP is an enzyme that in humans is encoded by the FAP gene. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Fibroblast activation protein, alpha/FAP using anti-Fibroblast activation protein, alpha/FAP antibody (A00422-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibroblast activation protein, alpha/FAP antigen affinity purified polyclonal antibody (Catalog # A00422-5) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibroblast activation protein, alpha/FAP at approximately 97 kDa. The expected band size for Fibroblast activation protein, alpha/FAP is at 95 kDa.

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Figure 2. Flow Cytometry analysis of SiHa cells using anti-Fibroblast activation protein, alpha/FAP antibody (A00422-5).
Overlay histogram showing SiHa cells stained with A00422-5 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibroblast activation protein, alpha/FAP Antibody (A00422-5, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For FAP (Source: Uniprot.org, NCBI)

Alternative Names

170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin FAP DPPIVA, FAPalpha, SIMP, FAP fibroblast activation protein alpha prolyl endopeptidase FAP|170 kDa melanoma membrane-bound gelatinase|dipeptidyl peptidase FAP|gelatine degradation protease FAP|integral membrane serine protease|post-proline cleaving enzyme|seprase|surface-expressed protease

For more info on FAP, check out the FAP Infographic

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In this infographic, you will see the following information for FAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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