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Product Info Summary
| SKU: | A04492 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-FKBP12 FKBP1A Antibody
SKU/Catalog Number
A04492
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FKBP12 FKBP1A Antibody (Catalog # A04492). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
FKBP12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-FKBP12 FKBP1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04492)
Host
Rabbit
Contents
FKBP12 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FKBP12 antibody was raised against an 18 amino acid peptide near the center of human FKBP12. The immunogen is located within the first 50 amino acids of FKBP12.
Cross-reactivity
FKBP12 antibody is human, mouse and rat reactive.
Reactive Species
A04492 is reactive to FKBP1A in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
12.0 kDa
Background of FKBP1A
FKBP, or FK506 binding protein, is a family of proteins that have prolyl isomerase activity and are related to the cyclophilins in function (1). FKBP12, also called FKBP1, was originally identified as a target of FK506 and rapamycin activity and catalyzes the transition between cis and transproline residues critical for proper folding of proteins (2). FKBP12 is an abundant, evolutionarily conserved cytoplasmic protein that is an important regulator of diverse array of cellular processes including T cell activation, entry into the cell cycle and intracellular calcium release (3-5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04492 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FKBP12 antibody can be used for detection of FKBP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FKBP12 in A431 cell lysate with FKBP12 antibody at 1 μg/ml.
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Immunocytochemistry of FKBP12 in A431 cells with FKBP12 antibody at 5 μg/mL.
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Immunofluorescence of FKBP12 in A431 cells with FKBP12 antibody at 20 μg/mL.
Specific Publications For Anti-FKBP12 FKBP1A Antibody (A04492)
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