Anti-FLIP CFLAR Antibody

Product Name

Anti-FLIP CFLAR Antibody

View all FLIP Antibodies

SKU/Catalog Number

A01295-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FLIP CFLAR Antibody (Catalog # A01295-2). Tested in ELISA, WB, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

FLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-FLIP CFLAR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01295-2)

Host

Rabbit

Contents

FLIP Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FLIP antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP. The sequence is identical in all FLIP splice variants. The immunogen is located within the first 50 amino acids of FLIP.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

FLIP recognizes all FLIP splice variants including FLIPa, b, and y.

Reactive Species

A01295-2 is reactive to CFLAR in Human, Mouse, Rat

Applications

A01295-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

55.344kDa

Background of FLIP

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FLIP antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of FLIP in (A) HeLa cell and (B) mouse liver tissue lysate with FLIP antibody at 1 μg/ml.

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Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 μg/mL.

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Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 μg/mL.

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Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 μg/mL.

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Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 μg/ml.

Red: FLIP Antibody (A01295-2)
Blue: DAPI staining

Gene/Protein Information For CFLAR (Source: Uniprot.org, NCBI)

Alternative Names

CASHFADD-like antiapoptotic molecule 1; CASP8 and FADD-like apoptosis regulator; CASP8AP1; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; CASPER; CasperFLAME1; Cellular FLICE-like inhibitory protein; CFLAR; c-FLIPR; c-FLIPS; CLARPMACH-related inducer of toxicity; FADD-like apoptotic molecule; FLAME; FLAME-1; FLIP; I-FLICE; I-FLICEMRITc-FLIPFLIPc-FLIPL; Inhibitor of FLICE; usurpin beta; Usurpin CFLAR CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, cFLIP CASP8 and FADD like apoptosis regulator CASP8 and FADD-like apoptosis regulator|FADD-like antiapoptotic molecule 1|MACH-related inducer of toxicity|caspase homolog|caspase-eight-related protein|caspase-like apoptosis regulatory protein|caspase-related inducer of apoptosis|cellular FLICE-like inhibitory protein|inhibitor of FLICE|testis secretory sperm-binding protein Li 233m|usurpin beta

For more info on CFLAR, check out the CFLAR Infographic

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In this infographic, you will see the following information for CFLAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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