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FLOT2 antibody (PB9623) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of RH35 cells using anti-Flotillin 2 antibody (PB9623).
Overlay histogram showing RH35 cells stained with PB9623 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Flotillin 2 Antibody (PB9623,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RH35 cells stained with PB9623 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Flotillin 2 Antibody (PB9623,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Flotillin 2/FLOT2 Antibody Picoband®
FLOT2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Tested Applications
FLOT2 antibody was positively detected by WB in:
human 293T whole cell, human Hela whole cell, human A549 whole cell, rat lung tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FLOT2 antibody was positively detected by IHC in:
human mammary cancer tissue, mouse intestine tissue, rat kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FLOT2 antibody was positively detected by IHC-F in:
rat kidney tissue, rat small intestine tissue, mouse kidney tissue
FLOT2 antibody was positively detected by ICC/IF in:
A431 cell
FLOT2 antibody was positively detected by FC in:
RH35 cell, U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Flotillin 2/FLOT2 Antibody Picoband®
View all FLOT2 Antibodies
View all FLOT2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse and rat Flotillin 2.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47-50 kDa
Calculated Molecular Weight
47.1 kDa
Background of FLOT2
FLOT2 (Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al. (1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al. (1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al. (2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.
Cite This Product
Anti-Flotillin 2/FLOT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9623)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Cow
99%
High Homology
Sheep
99%
High Homology
Zebrafish
78%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FLOT2 antibody
Immunohistochemistry (IHC) protocol for FLOT2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FLOT2 antibody
Flow Cytometry (FC) protocol for FLOT2 antibody
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Product Promise
We promise Anti-Flotillin 2/FLOT2 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9623
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026