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- Table of Contents
FLT4 antibody (A01276-3) performs well in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Flow Cytometry analysis of U20S cells using anti-VEGF Receptor 3 antibody (A01276-3).
Overlay histogram showing U20S cells stained with A01276-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGF Receptor 3 Antibody (A01276-3,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U20S cells stained with A01276-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGF Receptor 3 Antibody (A01276-3,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-VEGF Receptor 3/FLT4 Antibody Picoband®
FLT4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Tested Applications
FLT4 antibody was positively detected by WB in:
rat liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FLT4 antibody was positively detected by IHC in:
human lung cancer tissue, human placenta tissue, human prostatic cancer tissue, rat liver tissue, human rectal cancer tissue, mouse liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FLT4 antibody was positively detected by IHC-F in:
human placenta tissue
FLT4 antibody was positively detected by ICC/IF in:
A431 cell, A431 cell
FLT4 antibody was positively detected by FC in:
U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Human
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-VEGF Receptor 3/FLT4 Antibody Picoband®
View all FLT4 Antibodies
View all FLT4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human VEGF Receptor 3 recombinant protein (Position: Y25-N259).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
153 kDa
Calculated Molecular Weight
152.8 kDa
Background of FLT4
Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.
Cite This Product
Anti-VEGF Receptor 3/FLT4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01276-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
96%
High Homology
Rat
88%
Moderate Homology
Rabbit
87%
Moderate Homology
Sheep
87%
Moderate Homology
Cow
86%
Moderate Homology
Dog
86%
Moderate Homology
Mouse
86%
Moderate Homology
Zebrafish
38%
Low homology
Drosophila
24%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FLT4 antibody
Immunohistochemistry (IHC) protocol for FLT4 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FLT4 antibody
Flow Cytometry (FC) protocol for FLT4 antibody
Publications
Application:
Species:
Platelet activation relieves liver portal hypertension via the lymphatic system though the classical vascular endothelial growth factor receptor 3 signaling pathway
Published: 2025-03-14
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: IHC
Impact Factor: 5.40
Species: Rat
PMID: 40093669
Expression levels of VEGF‑C and VEGFR‑3 in renal cell carcinoma and their association with lymph node metastasis
Published: 2021-03-26
Journal: Experimental and Therapeutic Medicine
Application: IHC
Impact Factor: 2.45
Species: Human
PMID: 33850526
Expression of Angiopoietin-2 and Vascular Endothelial Growth Factor Receptor-3 Correlates with Lymphangiogenesis and Angiogenesis and Affects Survival of Oral Squamous Cell Carcinoma
Published: 2013-09-11
Journal: PLoS One
Application: IHC
Impact Factor: 3.73
Species: Human
PMID: 24040410
Interleukin-18 suppresses angiogenesis and lymphangiogenesis in implanted Lewis lung cancer
Published: 2010-11-26
Journal: Chinese Journal of Cancer Research
Application: ICC
Impact Factor: 0.20
Species: Rat
Lymphatic vessel density as a predictor of lymph node metastasis and its relationship with prognosis in urothelial carcinoma of the bladder
Published: 2010-09-30
Journal: BJU INTERNATIONAL
Application: IHC
Impact Factor: 2.87
Species: Human
PMID: 20883486
Antitumor activity of endogenous mFlt4 displayed on a T4 phage nanoparticle surface
Published: 2009-05-05
Journal: ACTA PHARMACOLOGICA SINICA
Application: IHC
Impact Factor: 1.68
Species: Mouse
PMID: 19417736
Expression of Cyclooxygenase-2 and Vascular Endothelial Growth Factor-C Correlates with Lymphangiogenesis and Lymphatic Invasion in Human Gastric Cancer
Published: 2007-10-24
Journal: ARCHIVES OF MEDICAL RESEARCH
Application: IHC
Impact Factor: 1.28
Species: Human
PMID: 18068001
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Product Promise
We promise Anti-VEGF Receptor 3/FLT4 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01276-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026