Effect of pretreatment with duloxetine on NAA-related enzyme expression in CeA in rats exposed to 6 h/3 h/0.5 h of WIRS. (A) Representative immunoblots of ASPA/GCP II/GCP III/NAAGSI/Nat8l performed in CeA lysates. Western blotting showed that duloxetine pretreatment had no effect on the expression level of these enzymes in CeA after 6 h of WIRS ( n = 5 in control, 5 mg/kg duloxetine and 20 mg/kg duloxetine group, n = 6 in 0 mg/kg duloxetine group). (B) WIRS for 3 h decreased the expression of GCP II and Nat8l in CeA, and duloxetine did not modulate the expression of these two enzymes ( n = 5 per group), while pretreatment with 20 mg/kg duloxetine increased NAAGSI and slightly increased GCP III expression in CeA exposed to WIRS, which did not change by WIRS ( n = 6 per group). (C) WIRS for 0.5 h decreased the expression of APSA in CeA, and pretreatment with 20 mg/kg duloxetine reversed this decrement ( n = 6 per group). ASPA/GCP II/GCP III/NAAGSI/Nat8l was normalized to β-actin. Values are expressed as means ± SEM. * p < 0.05, ** p < 0.01,*** p < 0.001, compared with control group, compared with control group, # p < 0.05, compared with WIRS + vehicle group.
Index in PubMed under a CC BY license. PMID: 35281914