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Product Info Summary
| SKU: | A15665 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Forkhead box protein D4-like 1 FoxD4L1 Antibody
SKU/Catalog Number
A15665
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Forkhead box protein D4-like 1 FoxD4L1 Antibody catalog # A15665. Tested in WB, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Forkhead box protein D4-like 1 FoxD4L1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15665)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from FoxD4L1 . at AA range: 320-400
Reactive Species
A15665 is reactive to FOXD4L1 in Human
Calculated molecular weight
43.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15665 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blot analysis of FOXD4L1 Antibody. The lane on the right is blocked with the FOXD4L1 peptide.
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Western Blot analysis of HuvEc cells using FoxD4L1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Specific Publications For Anti-Forkhead box protein D4-like 1 FoxD4L1 Antibody (A15665)
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Customer Q&As
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7 Customer Q&As for Anti-Forkhead box protein D4-like 1 FoxD4L1 Antibody
Question
Does anti-FoxD4L1 antibody A15665 work on monkey WB with testis?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
Our lab technicians have not tested anti-FoxD4L1 antibody A15665 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-FoxD4L1 antibody A15665 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-FoxD4L1 antibody A15665. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-21
Question
We are currently using anti-FoxD4L1 antibody A15665 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
The anti-FoxD4L1 antibody (A15665) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-12
Question
I see that the anti-FoxD4L1 antibody A15665 works with WB, what is the protocol used to produce the result images on the product page?
A. Yang
Verified customer
Asked: 2018-03-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-03-22
Question
Does anti-FoxD4L1 antibody A15665 work for WB with brain?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
According to the expression profile of brain, FOXD4L1 is highly expressed in brain. So, it is likely that anti-FoxD4L1 antibody A15665 will work for WB with brain.
Boster Scientific Support
Answered: 2017-09-14
Question
Do you have a BSA free version of anti-FoxD4L1 antibody A15665 available?
S. Taylor
Verified customer
Asked: 2016-01-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FoxD4L1 antibody A15665 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-01-28
Question
I have a question about product A15665, anti-FoxD4L1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Miller
Verified customer
Asked: 2014-04-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15665 anti-FoxD4L1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-04-16
