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Product Info Summary
| SKU: | M00723-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FOXP1 Monoclonal Antibody
SKU/Catalog Number
M00723-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FOXP1 Monoclonal Antibody catalog # M00723-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FOXP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00723-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFFD-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FOXP1 Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development.
Reactive Species
M00723-1 is reactive to FOXP1 in Human, Mouse, Rat
Observed Molecular Weight
75 kDa
Calculated molecular weight
75.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00723-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:50
Positive Control
WB: Daudi cell
Validation Images & Assay Conditions
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Western blot analysis of FOXP1 expression in Daudi cell lysate.
Specific Publications For Anti-FOXP1 Monoclonal Antibody (M00723-1)
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Customer Q&As
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6 Customer Q&As for Anti-FOXP1 Monoclonal Antibody
Question
I was wanting to use your anti-FOXP1 Monoclonal antibody for WB for mouse embryonic head tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryonic head tongue identification?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
As indicated on the product datasheet, M00723-1 anti-FOXP1 Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic head tongue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-22
Question
My lab would like to test anti-FOXP1 Monoclonal antibody M00723-1 on mouse embryonic head tongue for research purposes, then I may be interested in using anti-FOXP1 Monoclonal antibody M00723-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
The products we sell, including anti-FOXP1 Monoclonal antibody M00723-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-14
Question
Will M00723-1 anti-FOXP1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
You can see on the product datasheet, M00723-1 anti-FOXP1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-01
Question
Do you have a BSA free version of anti-FOXP1 Monoclonal antibody M00723-1 available?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FOXP1 Monoclonal antibody M00723-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-31
Question
Will anti-FOXP1 Monoclonal antibody M00723-1 work for WB with embryonic head tongue?
M. Miller
Verified customer
Asked: 2019-02-07
Answer
According to the expression profile of embryonic head tongue, FOXP1 is highly expressed in embryonic head tongue. So, it is likely that anti-FOXP1 Monoclonal antibody M00723-1 will work for WB with embryonic head tongue.
Boster Scientific Support
Answered: 2019-02-07
Question
Does anti-FOXP1 Monoclonal antibody M00723-1 work on bovine IF with lung?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
Our lab technicians have not validated anti-FOXP1 Monoclonal antibody M00723-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-FOXP1 Monoclonal antibody M00723-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine lung in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-06
