Click image to see more details
Product Info Summary
| SKU: | M02265 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Fragilis IFITM3 Monoclonal Antibody
SKU/Catalog Number
M02265
BM5362 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Fragilis IFITM3 Monoclonal Antibody catalog # M02265. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fragilis IFITM3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02265)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADAC-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Fragilis
Reactive Species
M02265 is reactive to IFITM3 in Human
Observed Molecular Weight
15 kDa
Calculated molecular weight
14.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02265 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Fragilis expression in HeLa cell lysate.
Specific Publications For Anti-Fragilis IFITM3 Monoclonal Antibody (M02265)
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Customer Q&As
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8 Customer Q&As for Anti-Fragilis IFITM3 Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02265?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Fragilis expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Will anti-Fragilis Monoclonal antibody M02265 work on horse WB with ectocervix?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
Our lab technicians have not validated anti-Fragilis Monoclonal antibody M02265 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Fragilis Monoclonal antibody M02265 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse ectocervix in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-11
Question
Do you have a BSA free version of anti-Fragilis Monoclonal antibody M02265 available?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Fragilis Monoclonal antibody M02265 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-21
Question
We are currently using anti-Fragilis Monoclonal antibody M02265 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The anti-Fragilis Monoclonal antibody (M02265) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-29
Question
Does anti-Fragilis Monoclonal antibody M02265 work for WB with brain?
S. Zhao
Verified customer
Asked: 2019-04-16
Answer
According to the expression profile of brain, IFITM3 is highly expressed in brain. So, it is likely that anti-Fragilis Monoclonal antibody M02265 will work for WB with brain.
Boster Scientific Support
Answered: 2019-04-16
Question
I was wanting to use your anti-Fragilis Monoclonal antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
L. Patel
Verified customer
Asked: 2019-03-15
Answer
You can see on the product datasheet, M02265 anti-Fragilis Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-15
Question
Is a blocking peptide available for product anti-Fragilis Monoclonal antibody (M02265)?
Verified Customer
Verified customer
Asked: 2018-08-24
Answer
We do provide the blocking peptide for product anti-Fragilis Monoclonal antibody (M02265). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-08-24
Question
Can you help my question with product M02265, anti-Fragilis Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02265 anti-Fragilis Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-30
