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Product Info Summary
| SKU: | A09605 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-FREM1 Antibody
SKU/Catalog Number
A09605
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FREM1 Antibody (Catalog # A09605). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
FREM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-FREM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09605)
Host
Rabbit
Contents
FREM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FREM1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human FREM1. The immunogen is located within the last 50 amino acids of FREM1.
Reactive Species
A09605 is reactive to FREM1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
244.2 kDa
Background of FREM1
FREM1 is a member of the FRAS1-related extracellular matrix protein family and is thought to play a role in craniofacial and renal development. FREM1 functions as an extracellular matrix protein that is essential for epidermal adhesion during embryogenesis and may also participate in epidermal differentiation. It is recognized by cells in the embryonic skin and hair follicles through different members of the integrin family. Deficiency in the Fras1/Frem genes gives rise to the bleb phenotype, which is equivalent to the human hereditary disorder Fraser syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09605 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FREM1 antibody can be used for detection of FREM1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1μg/mL.
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Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.
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Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.
Specific Publications For Anti-FREM1 Antibody (A09605)
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