Product Info Summary
SKU: | A04292Y500 |
---|---|
Size: | 100 ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Frizzled-5 (Y500) FZD5 Antibody
View all Frizzled-5 Antibodies
SKU/Catalog Number
A04292Y500
Size
100 ul
Form
Liquid
Description
Boster Bio Anti-Frizzled-5 (Y500) FZD5 Antibody catalog # A04292Y500. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Frizzled-5 (Y500) FZD5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04292Y500)
Host
Rabbit
Contents
Rabbit IgG, 1 mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 468-519 of Human Frizzled-5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04292Y500 is reactive to FZD5 in Human, Mouse, Rat
Applications
A04292Y500 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
64.507kDa
Background of Frizzled-5
Transcription factor. Activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling. Induces apoptosis.
Cook T., J. Biol. Chem. 273:25929-25936(1998).
Asano H., Mol. Cell. Biol. 19:3571-3579(1999).
Neve B., Proc. Natl. Acad. Sci. U.S.A. 102:4807-4812(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot (WB) analysis of Frizzled-5 (Y500) pAb at 1:500 dilution
Lane1:PC12 whole cell lysate(40 ug)
Lane2:MEF whole cell lysate(40 ug)
Lane3:HEK293T whole cell lysate(40 ug)
Lane4:HepG2 whole cell lysate(40 ug)
Lane5:K562 whole cell lysate(40 ug)
Protein Target Info & Infographic
Gene/Protein Information For FZD5 (Source: Uniprot.org, NCBI)
Gene Name
FZD5
Full Name
Frizzled-5
Weight
64.507kDa
Superfamily
G-protein coupled receptor Fz/Smo family
Alternative Names
C2orf31; chromosome 2 open reading frame 31; DKFZP434E2135; frizzled (Drosophila) homolog 5; frizzled homolog 5 (Drosophila); Frizzled5; Frizzled-5; Frz-5; FZ-5; FZD5; fzE5; HFZ5; MGC129692; seven-transmembrane receptor frizzled-5; Wnt receptor FZD5 C2orf31, HFZ5 frizzled class receptor 5 frizzled-5|Wnt receptor|frizzled 5, seven transmembrane spanning receptor|frizzled family receptor 5|fz-5|fzE5|seven-transmembrane receptor frizzled-5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FZD5, check out the FZD5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FZD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Frizzled-5 (Y500) FZD5 Antibody (A04292Y500)
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No publications found for A04292Y500
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