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Product Info Summary
| SKU: | A04075 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Tyrosine-protein kinase FRK FRK Antibody
SKU/Catalog Number
A04075
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Tyrosine-protein kinase FRK FRK Antibody (Catalog # A04075). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tyrosine-protein kinase FRK FRK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04075)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human FRK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04075 is reactive to FRK in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
Calculated molecular weight
58254 MW
Background of FRK
Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
Lee J., Gene 138:247-251(1994).
Cance W.G., Cell Growth Differ. 5:1347-1355(1994).
Zhang Y., Mol. Cell. Proteomics 4:1240-1250(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04075 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Positive Control
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FRK antibody A04075.
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Western blot analysis of extracts from Jurkat cells, using FRK antibody A04075.
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Immunofluorescence analysis of HUVEC cells, using FRK antibody A04075.
Protein Target Info & Infographic
Gene/Protein Information For FRK (Source: Uniprot.org, NCBI)
Gene Name
FRK
Full Name
Tyrosine-protein kinase FRK
Weight
58254 MW
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.10; Frk; fyn-related kinaseProtein-tyrosine kinase 5; Gtk; GTKEC 2.7.10.2; Nuclear tyrosine protein kinase RAK; PTK5 protein tyrosine kinase 5; PTK5; Rak; RAKPTK5; tyrosine-protein kinase FRK FRK GTK, PTK5, RAK fyn related Src family tyrosine kinase tyrosine-protein kinase FRK|PTK5 protein tyrosine kinase 5|fyn-related kinase|nuclear tyrosine protein kinase RAK|protein-tyrosine kinase 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FRK, check out the FRK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FRK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tyrosine-protein kinase FRK FRK Antibody (A04075)
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No publications found for A04075
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Tyrosine-protein kinase FRK FRK Antibody
Question
Does A04075 anti-FRK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
As indicated on the product datasheet, A04075 anti-FRK antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-04
Question
See attached the WB image, lot number and protocol we used for liver using anti-FRK antibody A04075. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-19
Question
We are currently using anti-FRK antibody A04075 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The anti-FRK antibody (A04075) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
Is this A04075 anti-FRK antibody reactive to the isotypes of FRK?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The immunogen of A04075 anti-FRK antibody is Synthesized peptide derived from internal of human FRK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-06
Question
Would anti-FRK antibody A04075 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
According to the expression profile of liver, FRK is highly expressed in liver. So, it is likely that anti-FRK antibody A04075 will work for WB with liver.
Boster Scientific Support
Answered: 2019-10-30
Question
Is there a BSA free version of anti-FRK antibody A04075 available?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FRK antibody A04075 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-FRK antibody A04075. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-16
Question
Is a blocking peptide available for product anti-FRK antibody (A04075)?
E. Rodriguez
Verified customer
Asked: 2018-07-11
Answer
We do provide the blocking peptide for product anti-FRK antibody (A04075). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-07-11
Question
Does anti-FRK antibody A04075 work on zebrafish IF with testis?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
Our lab technicians have not tested anti-FRK antibody A04075 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-FRK antibody A04075 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish testis in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-03
Question
I was wanting to use to test anti-FRK antibody A04075 on mouse liver for research purposes, then I may be interested in using anti-FRK antibody A04075 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
The products we sell, including anti-FRK antibody A04075, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-28
Question
I was wanting to use your anti-FRK antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
A. Kulkarni
Verified customer
Asked: 2017-02-20
Answer
As indicated on the product datasheet, A04075 anti-FRK antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-20
Question
I see that the anti-FRK antibody A04075 works with WB, what is the protocol used to produce the result images on the product page?
Z. Patel
Verified customer
Asked: 2014-04-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-04-28
Question
Can you help my question with product A04075, anti-FRK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
W. Gonzalez
Verified customer
Asked: 2013-03-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04075 anti-FRK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-03-13
