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Product Info Summary
| SKU: | PB9372 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-FRZB Antibody Picoband™
View all sFRP-3/FRZB Antibodies
SKU/Catalog Number
PB9372
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FRZB Antibody Picoband™ catalog # PB9372. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FRZB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9372)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FRZB recombinant protein (Position: E176-N325). Human FRZB shares 90% amino acid (aa) sequence identity with mouse FRZB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9372 is reactive to FRZB in Human
Applications
PB9372 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
36 kDa
Calculated molecular weight
36.254kDa
Background of sFRP-3/FRZB
FRZB is also known as FRE or OS1. The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. FRZB is a Wnt-binding protein especially important in embryonic development. It is a competitor for the cell-surface G-protein receptor Frizzled. FRZB is localized in the extracellular plasma membrane. Unlike frizzled, frzb lacks the 7 transmembrane domains normally found in G-protein-coupled receptors. It is still considered a homolog of frizzled because it contains a Cysteine Rich Domain (CRD), and because of its intracellular C-terminus which is crucial for signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, -
ELISA , 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-FRZB Picoband antibody, PB9372, Western blotting
All lanes: Anti FRZB (PB9372) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
Protein Target Info & Infographic
Gene/Protein Information For FRZB (Source: Uniprot.org, NCBI)
Gene Name
FRZB
Full Name
Secreted frizzled-related protein 3
Weight
36.254kDa
Superfamily
secreted frizzled-related protein (sFRP) family
Alternative Names
FIZ; FREOS1; Frezzled; Fritz; Frizzled-related protein 1; frizzled-related protein; FRP; FRP-3; FRZB; Frzb1; FRZB-1; FRZB1sFRP-3; FRZB-PEN; FZRB; hFIZ; secreted frizzled-related protein 3; sFRP3; sFRP-3; SFRP3frezzled; SRFP3frizzled homolog-related FRZB FRE, FRITZ, FRP-3-1, FRZB-PEN, FRZB1, FZRB, OS1, SFRP3, SRFP3, hFIZ, FRZB frizzled related protein secreted frizzled-related protein 3|frezzled|frizzled homolog-related|frizzled-related protein 1|sFRP-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FRZB, check out the FRZB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FRZB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FRZB Antibody Picoband™ (PB9372)
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No publications found for PB9372
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-FRZB Antibody Picoband™
Question
We are currently using anti-FRZB antibody PB9372 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
S. Patel
Verified customer
Asked: 2019-11-26
Answer
The anti-FRZB antibody (PB9372) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-26
Question
I am looking for to test anti-FRZB antibody PB9372 on human embryo for research purposes, then I may be interested in using anti-FRZB antibody PB9372 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Mitchell
Verified customer
Asked: 2019-10-31
Answer
The products we sell, including anti-FRZB antibody PB9372, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-31
Question
Will anti-FRZB antibody PB9372 work on horse WB with pancreas spleen?
Verified Customer
Verified customer
Asked: 2017-09-27
Answer
Our lab technicians have not validated anti-FRZB antibody PB9372 on horse. You can run a BLAST between horse and the immunogen sequence of anti-FRZB antibody PB9372 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse pancreas spleen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-27
Question
I was wanting to use your anti-FRZB antibody for ELISA for human embryo on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human embryo identification?
G. Banerjee
Verified customer
Asked: 2017-02-13
Answer
It shows on the product datasheet, PB9372 anti-FRZB antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human embryo in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-13
