Anti-FSH Receptor/FSHR Antibody Picoband™

Product Name

Anti-FSH Receptor/FSHR Antibody Picoband™

View all FSHR Antibodies

SKU/Catalog Number

PB10065

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FSH Receptor/FSHR Antibody Picoband™ catalog # PB10065. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FSH Receptor/FSHR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10065)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human FSH Receptor recombinant protein (Position: C18-N187). Human FSH Receptor shares 91.2% and 90% amino acid (aa) sequence identity with mouse and rat FSH Receptor, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10065 is reactive to FSHR in Human, Mouse, Rat

Applications

PB10065 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

78 kDa

Calculated molecular weight

78.265kDa

Background of FSHR

The follicle-stimulating hormone receptor or FSH receptor (FSHR) is a transmembrane receptor that interacts with the follicle-stimulating hormone (FSH) and represents a G protein-coupled receptor (GPCR). This FSHR gene is mapped to chromosome 2p21 by fluorescence in situ hybridization. The protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Mutations in this gene cause ovarian dysgenesis type 1, and also ovarian hyperstimulation syndrome. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FSH Receptor using anti-FSH Receptor antibody (PB10065).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates，
Lane 2: rat ovary tissue lysates，
Lane 3: mouse testis tissue lysates，
Lane 4: mouse ovary tissue lysates,
Lane 5: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSH Receptor antigen affinity purified polyclonal antibody (Catalog # PB10065) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSH Receptor at approximately 78 kDa. The expected band size for FSH Receptor is at 78 kDa.

Gene/Protein Information For FSHR (Source: Uniprot.org, NCBI)

Alternative Names

follicle stimulating hormone receptor; follicle-stimulating hormone receptor; Follitropin receptor; FSH R; FSH receptor; FSHR; FSH-R; LGR1; LGR1FSHRO; MGC141667; MGC141668; ODG1 FSHR FSHR1O, LGR1, ODG1, FSHR follicle stimulating hormone receptor follicle-stimulating hormone receptor|FSH receptor|follitropin receptor

For more info on FSHR, check out the FSHR Infographic

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In this infographic, you will see the following information for FSHR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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