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Product Info Summary
| SKU: | M06541 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FSP1/AIFM2 Antibody (Monoclonal, 34A43)
SKU/Catalog Number
M06541
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-FSP1/AIFM2 Antibody (Monoclonal, 34A43) catalog # M06541. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FSP1/AIFM2 Antibody (Monoclonal, 34A43) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06541)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
34A43
Immunogen
Recombinant protein within human AMID aa 3-371.
Reactive Species
M06541 is reactive to AIFM2 in Human, Mouse, Rat
Observed Molecular Weight
41 kDa
Calculated molecular weight
40.5 kDa
Background of AIFM2
This gene encodes a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06541 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
Flow Cytometry (Fixed), 1:50-200
Positive Control
WB: human HepG2 whole cell, human A549 whole cell, human CACO-2 whole cell, human U251 whole cell, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue
IHC: human stomach cancer tissue, human liver cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of FSP1/AIFM2 using anti-FSP1/AIFM2 antibody (M06541).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSP1/AIFM2 antigen affinity purified monoclonal antibody (M06541) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FSP1/AIFM2 at approximately 41 kDa. The expected band size for FSP1/AIFM2 is at 41 kDa.
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IHC analysis of FSP1/AIFM2/MRC1 using anti-FSP1/AIFM2/MRC1 antibody (M06541).
FSP1/AIFM2/MRC1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FSP1/AIFM2/MRC1 Antibody (M06541) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of FSP1 using anti-FSP1 antibody (M06541).
FSP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FSP1 Antibody (M06541) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-FSP1/AIFM2 Antibody (Monoclonal, 34A43) (M06541)
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