Product Info Summary
SKU: | A12417 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Fucokinase FUK Monoclonal Antibody
SKU/Catalog Number
A12417
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Fucokinase FUK Monoclonal Antibody catalog # A12417. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fucokinase FUK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12417)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
6E2
Isotype
IgG
Immunogen
Purified recombinant fragment of human Fucokinase expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12417 is reactive to FCSK in Human, Monkey, Mouse, Rat
Applications
A12417 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
117.623kDa
Background of FCSK
COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
FC, 1:200-1:400
Validation Images & Assay Conditions
Click image to see more details
Flow cytometric (FCM) analysis of HeLa cells using Fucokinase Monoclonal antibody (green) and negative control (purple).
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Western Blot (WB) analysis using Fucokinase Monoclonal antibody against HeLa (1), HepG2 (2), Jurkat (3), A431 (4), HEK293 (5), MCF-7 (6), PC-12 (7), Cos7 (8), and NIH/3T3 (9) cell lysate.
Click image to see more details
ELISA analysis of Fucokinase antibody.
Protein Target Info & Infographic
Gene/Protein Information For FCSK (Source: Uniprot.org, NCBI)
Gene Name
FCSK
Full Name
L-fucose kinase
Weight
117.623kDa
Superfamily
GHMP kinase family
Alternative Names
L-fucose kinase FCSK 1110046B12Rik, CDGF2, FUK fucose kinase L-fucose kinase|fucokinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCSK, check out the FCSK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCSK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fucokinase FUK Monoclonal Antibody (A12417)
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No publications found for A12417
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Fucokinase FUK Monoclonal Antibody
Question
Our lab want to know about using your anti-Fucokinase Monoclonal antibody for response to dopamine studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
We appreciate your inquiry. This A12417 anti-Fucokinase Monoclonal antibody is validated on hela cells. It is guaranteed to work for Flow Cytometry, WB in human, monkey, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-16