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Product Info Summary
| SKU: | PB9941 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GRO gamma/CXCL3 Antibody Picoband®
SKU/Catalog Number
PB9941
PB0994 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GRO gamma/CXCL3 Antibody Picoband® catalog # PB9941. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRO gamma/CXCL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9941)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse GRO gamma recombinant protein (Position: S32-S100). Mouse GRO gamma shares 60.6% and 90.6% amino acid (aa) sequence identity with human and rat GRO gamma, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9941 is reactive to CXCL3 in Mouse
Observed Molecular Weight
25 kDa
Calculated molecular weight
10.7 kDa
Background of CXCL3
Chemokine (C-X-C motif) ligand 3 (CXCL3), also known as GRO protein gamma (GROg) and macrophage inflammatory protein-2-beta (MIP2b), is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9941 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse lung tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of GRO gamma using anti-GRO gamma antibody (PB9941).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates,
Lane 2: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRO gamma antigen affinity purified polyclonal antibody (Catalog # PB9941) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRO gamma at approximately 25 kDa. The expected band size for GRO gamma is at 11 kDa.
Specific Publications For Anti-GRO gamma/CXCL3 Antibody Picoband® (PB9941)
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1 Customer Q&As for Anti-GRO gamma/CXCL3 Antibody Picoband®
Question
We are currently using anti-GRO gamma/CXCL3 antibody PB9941 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
The anti-GRO gamma/CXCL3 antibody (PB9941) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-13
