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Product Info Summary
| SKU: | M00771 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FUS / TLS Rabbit Monoclonal Antibody
SKU/Catalog Number
M00771
BM4857 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FUS / TLS Rabbit Monoclonal Antibody catalog # M00771. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FUS / TLS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00771)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IHC-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FUS / TLS
Reactive Species
M00771 is reactive to FUS in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
53.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00771 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human K562 whole cell, human HepG2 whole cell, human Hela whole cell, human Jurkat whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of FUS using anti-FUS antibody (M00771).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FUS antigen affinity purified monoclonal antibody (M00771) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FUS at approximately 70 kDa. The expected band size for FUS is at 70 kDa.
Specific Publications For Anti-FUS / TLS Rabbit Monoclonal Antibody (M00771)
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Customer Q&As
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16 Customer Q&As for Anti-FUS / TLS Rabbit Monoclonal Antibody
Question
Would M00771 anti-FUS / TLS Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
You can see on the product datasheet, M00771 anti-FUS / TLS Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-07
Question
We are currently using anti-FUS / TLS Rabbit Monoclonal antibody M00771 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The anti-FUS / TLS Rabbit Monoclonal antibody (M00771) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-04
Question
I was wanting to use your anti-FUS / TLS Rabbit Monoclonal antibody for IF for mouse colon carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse colon carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
You can see on the product datasheet, M00771 anti-FUS / TLS Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-FUS / TLS Rabbit Monoclonal antibody M00771. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-10
Question
I see that the anti-FUS / TLS Rabbit Monoclonal antibody M00771 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-04
Question
Is there a BSA free version of anti-FUS / TLS Rabbit Monoclonal antibody M00771 available?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FUS / TLS Rabbit Monoclonal antibody M00771 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-22
Question
Our team were content with the WB result of your anti-FUS / TLS Rabbit Monoclonal antibody. However we have observed positive staining in colon carcinoma nucleus using this antibody. Is that expected? Could you tell me where is FUS supposed to be expressed?
T. Banerjee
Verified customer
Asked: 2019-09-11
Answer
From literature, colon carcinoma does express FUS. Generally FUS expresses in nucleus. Regarding which tissues have FUS expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 24129315
Erythroleukemia, Pubmed ID: 23186163
Fetal brain cortex, Pubmed ID: 8187069, 9660765
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Lymph, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-11
Question
Can you help my question with product M00771, anti-FUS / TLS Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00771 anti-FUS / TLS Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-15
Question
I am interested in using your anti-FUS / TLS Rabbit Monoclonal antibody for protein homooligomerization studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-01
Answer
We appreciate your inquiry. This M00771 anti-FUS / TLS Rabbit Monoclonal antibody is tested on k562 cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-01
Question
Would anti-FUS / TLS Rabbit Monoclonal antibody M00771 work for IF with colon carcinoma?
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
According to the expression profile of colon carcinoma, FUS is highly expressed in colon carcinoma. So, it is likely that anti-FUS / TLS Rabbit Monoclonal antibody M00771 will work for IF with colon carcinoma.
Boster Scientific Support
Answered: 2018-09-21
Question
We have observed staining in rat colon carcinoma. Are there any suggestions? Is anti-FUS / TLS Rabbit Monoclonal antibody supposed to stain colon carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
According to literature colon carcinoma does express FUS. According to Uniprot.org, FUS is expressed in right testis, lung lymph, fetal brain cortex, leukemic t-cell, erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have FUS expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 24129315
Erythroleukemia, Pubmed ID: 23186163
Fetal brain cortex, Pubmed ID: 8187069, 9660765
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Lymph, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-02-26
Question
We have tried in the past anti-FUS / TLS Rabbit Monoclonal antibody for WB on right testis a few months ago. I am using rat, and We are going to use the antibody for IF next. I am interested in examining right testis as well as lung lymph in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
N. Banerjee
Verified customer
Asked: 2017-01-04
Answer
I took a look at the website and datasheets of our anti-FUS / TLS Rabbit Monoclonal antibody and it seems that M00771 has been tested on rat in both WB and IF. Thus M00771 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-01-04
Question
We want to test anti-FUS / TLS Rabbit Monoclonal antibody M00771 on mouse colon carcinoma for research purposes, then I may be interested in using anti-FUS / TLS Rabbit Monoclonal antibody M00771 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Krishna
Verified customer
Asked: 2016-09-15
Answer
The products we sell, including anti-FUS / TLS Rabbit Monoclonal antibody M00771, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-09-15
Question
Here is the WB image, lot number and protocol we used for colon carcinoma using anti-FUS / TLS Rabbit Monoclonal antibody M00771. Please let me know if you require anything else.
G. Anderson
Verified customer
Asked: 2015-01-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-06
Question
Is a blocking peptide available for product anti-FUS / TLS Rabbit Monoclonal antibody (M00771)?
T. Krishna
Verified customer
Asked: 2013-11-11
Answer
We do provide the blocking peptide for product anti-FUS / TLS Rabbit Monoclonal antibody (M00771). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-11-11
Question
Is this M00771 anti-FUS / TLS Rabbit Monoclonal antibody reactive to the isotypes of FUS?
K. Gonzalez
Verified customer
Asked: 2013-10-07
Answer
The immunogen of M00771 anti-FUS / TLS Rabbit Monoclonal antibody is A synthesized peptide derived from human FUS / TLS. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-10-07
