Anti-FUT3/Fuct Iii Antibody

Product Name

Anti-FUT3/Fuct Iii Antibody

View all FUT3 Antibodies

SKU/Catalog Number

A03774

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-FUT3/Fuct Iii Antibody (Catalog # A03774). Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FUT3/Fuct Iii Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03774)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human FUT3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03774 is reactive to FUT3 in Human

Applications

A03774 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

42.117kDa

Background of FUT3

May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.

Kukowska-Latallo J.F., Genes Dev. 4:1288-1303(1990).
Cameron H.S., J. Biol. Chem. 270:20112-20122(1995).
Rahim I., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

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Western blot analysis of extracts from K562 cells and Jurkat cells, using MRPS7 antibody A03774.

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MRPS7 antibody A03774.

Gene/Protein Information For FUT3 (Source: Uniprot.org, NCBI)

Alternative Names

CD174; FT3B; Fucosyltransferase 3; FucT-III; FUT3; Les; Lewis FT FUT3 CD174, FT3B, FucT-III, LE, Les fucosyltransferase 3 (Lewis blood group) 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3|Lewis FT|alpha-(1,3/1,4)-fucosyltransferase|alpha-3-fucosyltransferase FUT3|blood group Lewis alpha-4-fucosyltransferase|fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)|fucosyltransferase III|galactoside 3(4)-L-fucosyltransferase|truncated fucosyltransferase 3

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