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Product Info Summary
| SKU: | A01380 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ME2 Antibody
SKU/Catalog Number
A01380
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ME2 Antibody (Catalog # A01380). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
ME2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ME2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01380)
Host
Rabbit
Contents
ME2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ME2 antibody was raised against a 17 amino acid peptide near the center of human ME2. The immunogen is located within amino acids 340 - 390 of ME2.
Cross-reactivity
ME2 antibody is human specific. At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1.
Reactive Species
A01380 is reactive to ME2 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
65.4 kDa
Background of ME2
ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate (1). It is related to malic enzyme 1 (ME1), a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (2). The expression of both malic enzymes is reciprocally regulated by p53; this regulation has been shown to modulate metabolism and senescence (3). Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01380 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ME2 antibody can be used for detection of ME2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2μg/ml.
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Immunohistochemistry of ME2 in human spleen tissue with ME2 antibody at 5 μg/mL.
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Immunofluorescence of ME2 in human spleen tissue with ME2 antibody at 20 μg/mL.
Specific Publications For Anti-ME2 Antibody (A01380)
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