Anti-GABA A Receptor alpha 1/GABRA1 Antibody

Product Name

Anti-GABA A Receptor alpha 1/GABRA1 Antibody

View all GABA-AR alpha 1 Antibodies

SKU/Catalog Number

PA1578

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GABA A Receptor alpha 1/GABRA1 Antibody catalog # PA1578. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GABA A Receptor alpha 1/GABRA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1578)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GABA A Receptor alpha 1, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1578 is reactive to GABRA1 in Human, Mouse, Rat

Applications

PA1578 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

51.802kDa

Background of GABA-AR alpha 1

GABRA1, Gamma-aminobutyric acid receptor subunit alpha-1, is a protein that in humans is encoded by the GABRA1 gene. The 1,055-bp GABRA1 clone contained an open reading frame and 260 nucleotides in the 5-prime noncoding region. The 351-amino acid sequence shows 97% homology with its bovine counterpart. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

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Anti-GABA A Receptor alpha 1 antibody, PA1578, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate

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Anti-GABA A Receptor alpha 1 antibody, PA1578, IHC(P)
IHC(P): Rat Brain Tissue

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Figure 3. Western blot analysis of GABRA1 using anti-GABRA1 antibody (PA1578).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABRA1 antigen affinity purified polyclonal antibody (Catalog # PA1578) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABRA1 at approximately 52KD. The expected band size for GABRA1 is at 52KD.

Gene/Protein Information For GABRA1 (Source: Uniprot.org, NCBI)

Alternative Names

ECA4; EJM; EJM5GABA(A) receptor subunit alpha-1; GABA A R alpha 1; GABAAR alpha 1; GABAARa1; GABRA1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; gamma-aminobutyric acid receptor subunit alpha-1 GABRA1 DEE19, ECA4, EIEE19, EJM, EJM5 gamma-aminobutyric acid type A receptor subunit alpha1 gamma-aminobutyric acid receptor subunit alpha-1|GABA(A) receptor subunit alpha-1|GABA(A) receptor, alpha 1|gamma-aminobutyric acid (GABA) A receptor, alpha 1|gamma-aminobutyric acid type A receptor alpha1 subunit

For more info on GABRA1, check out the GABRA1 Infographic

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In this infographic, you will see the following information for GABRA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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