Click image to see more details
Product Info Summary
| SKU: | A09362 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GABAA R epsilon GABRE Antibody
View all GABA Receptor Epsilon Antibodies
SKU/Catalog Number
A09362
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GABAA R epsilon GABRE Antibody catalog # A09362. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GABAA R epsilon GABRE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09362)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
1A5
Isotype
IgG
Immunogen
The immunogen is a synthetic peptide directed towards the C-terminal region of Human C20orf72
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09362 is reactive to GABRE in Human
Applications
A09362 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
57.972kDa
Background of GABA Receptor Epsilon
APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of extracts from Jurkat cells, using GABAA Repsilon Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For GABRE (Source: Uniprot.org, NCBI)
Gene Name
GABRE
Full Name
Gamma-aminobutyric acid receptor subunit epsilon
Weight
57.972kDa
Superfamily
ligand-gated ion channel (TC 1.A.9) family
Alternative Names
GABA A R epsilon; GABA(A) receptor subunit epsilon; GABAAR epsilon; GABAARe; gamma-aminobutyric acid (GABA) A receptor, epsilon; gamma-aminobutyric acid receptor subunit epsilon GABRE gamma-aminobutyric acid type A receptor subunit epsilon gamma-aminobutyric acid receptor subunit epsilon|GABA(A) receptor subunit epsilon|GABA(A) receptor, epsilon|gamma-aminobutyric acid (GABA) A receptor, epsilon|gamma-aminobutyric acid type A receptor epsilon subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GABRE, check out the GABRE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GABRE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GABAA R epsilon GABRE Antibody (A09362)
Hello CJ!
No publications found for A09362
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GABAA R epsilon GABRE Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-GABAA R epsilon GABRE Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-GABAA R epsilon GABRE Antibody
Question
We are currently using anti-GABAA R epsilon antibody A09362 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
The anti-GABAA R epsilon antibody (A09362) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-19
Question
My question regarding product A09362, anti-GABAA R epsilon antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09362 anti-GABAA R epsilon antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-18
Question
Does anti-GABAA R epsilon antibody A09362 work on canine WB with brain?
Verified Customer
Verified customer
Asked: 2018-03-21
Answer
Our lab technicians have not validated anti-GABAA R epsilon antibody A09362 on canine. You can run a BLAST between canine and the immunogen sequence of anti-GABAA R epsilon antibody A09362 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-21
Question
I see that the anti-GABAA R epsilon antibody A09362 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-06
Question
Do you have a BSA free version of anti-GABAA R epsilon antibody A09362 available?
H. Anderson
Verified customer
Asked: 2017-02-20
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GABAA R epsilon antibody A09362 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-02-20
Question
I was wanting to use your anti-GABAA R epsilon antibody for WB for human subcutaneous adipose tissue on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human subcutaneous adipose tissue identification?
D. Evans
Verified customer
Asked: 2016-07-26
Answer
As indicated on the product datasheet, A09362 anti-GABAA R epsilon antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human subcutaneous adipose tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-07-26
Question
See attached the WB image, lot number and protocol we used for subcutaneous adipose tissue using anti-GABAA R epsilon antibody A09362. Please let me know if you require anything else.
M. Wu
Verified customer
Asked: 2014-06-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-26
