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CD44 antibody (A00052) performs well in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 63 Publications

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a00052 cd44 primary antibodies fc testing 6

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Flow Cytometry analysis of Jurkat cells using anti-CD44 antibody (A00052).
Overlay histogram showing Jurkat cells stained with A00052 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD44 Antibody (A00052,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-CD44 Antibody Picoband®

Name: Anti-CD44 Antibody Picoband®
Brand: Boster Bio
SKU: A00052
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

CD44 antibody

63 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Tested Applications

CD44 antibody was positively detected by WB in:

human U251 whole cell, rat lung tissue, rat small intestine tissue, mouse lung tissue tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CD44 antibody was positively detected by IHC in:

human colon cancer tissue, human placenta tissue, rat kidney tissue, mouse kidney tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CD44 antibody was positively detected by IF in:

mouse lymphaden tissue, mouse lymphaden tissue

CD44 antibody was positively detected by FC in:

Jurkat cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-CD44 Antibody Picoband®
View all CD44 Antibodies

Form

Lyophilized

Uniprot

P16070

Gene ID

960

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human CD44 recombinant protein (Position: Q21-H259).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

82 kDa

Calculated Molecular Weight

81.5 kDa

Background of CD44

CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Cite This Product

Anti-CD44 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00052)

Protocols

Western Blot protocol for CD44 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CD44 antibody

Review Protocol

Immunofluorescence (IF) protocol for CD44 antibody

Review Protocol

Flow Cytometry (FC) protocol for CD44 antibody

Review Protocol

Publications

KCTD15 Enhances Stem Cell‐Like Properties and Promotes Triple‐Negative Breast Cancer Progression Through KLF4/β‐Catenin Signaling

Published: 2026-02-16

Journal: FASEB JOURNAL

Application: WB

Impact Factor: 4.20

Species: Human

PMID: 41693649

SKU	A00052
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Cyclin A2/CCNA2 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows a schematic of Western blot results for the target protein CCNA2 and the internal control Actin in rat colon across different groups. Expression was increased in the model group. Among the low, medium, and high doses of the herbal treatment, the low-dose group showed the best effect. The target bands are clear, and the experimental results are satisfactory.

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Anti-CD44 Antibody Picoband®

CD44 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

KCTD15 Enhances Stem Cell‐Like Properties and Promotes Triple‐Negative Breast Cancer Progression Through KLF4/β‐Catenin Signaling

Effects of intermittent hypoxia on adipose-derived mesenchymal stem cells in protecting alveolar type II cells from injury

A self-amplifying cuproptosis nanomedicine to overcome immunosuppression by blocking tumor-derived exosomes for enhancing lung cancer immunotherapy

Periodic asymmetric field enhances electrofusion of nanoscale lipid systems

Lung Cancer Cell-intrinsic Asparagine Synthetase Potentiates Anti-Tumor Immunity via Modulating Immunogenicity and Facilitating Immune Remodeling in Metastatic Tumor-draining Lymph Nodes

Identification of the Oncogenic Role of MSH2 in the Stemness and Progression of Glioma Through Regulating Wnt Signaling Pathway

The R-RAS2 GTPase is a signaling hub in triple-negative breast cancer cell metabolism and metastatic behavior

Tubular CD44 plays a key role in aggravating AKI through NF-κB p65-mediated mitochondrial dysfunction

MiR-21-5p Modulates Cisplatin-Resistance of CD44+ Gastric Cancer Stem Cells Through Regulating the TGF-β2/SMAD Signaling Pathway

Aronia melanocarpa L. fruit peels show anti-cancer effects in preclinical models of breast carcinoma: The perspectives in the chemoprevention and therapy modulation

Hematopoietic stem and progenitor cell membrane-coated vesicles for bone marrow-targeted leukaemia drug delivery

Precisely targeted drug delivery by mesenchymal stem cells-based biomimetic liposomes to cerebral ischemia-reperfusion injured hemisphere

Exploring miR-21 as a key regulator in two distinct approaches of bone marrow stromal cells differentiation into Schwann-like cells

m6A-methylated KCTD21-AS1 regulates macrophage phagocytosis through CD47 and cell autophagy through TIPR

Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration

Effects of norepinephrine‑induced activation of rat vascular adventitial fibroblasts on proliferation and migration of BMSCs involved in vascular remodeling

MiR-31 improves spinal cord injury in mice by promoting the migration of bone marrow mesenchymal stem cells

Pentagalloylglucose suppresses the growth and migration of human nasopharyngeal cancer cells via the GSK3β/β-catenin pathway in vitro and in vivo

β-catenin-controlled tubular cell-derived exosomes play a key role in fibroblast activation via the OPN-CD44 axis

Identification of Key Biomarkers and Pathways for Maintaining Cognitively Normal Brain Aging Based on Integrated Bioinformatics Analysis

LncRNA LINC00662 Exerts an Oncogenic Effect on Osteosarcoma by the miR-16-5p/ITPR1 Axis

Chemopreventive and Therapeutic Efficacy of Cinnamomum zeylanicum L. Bark in Experimental Breast Carcinoma: Mechanistic In Vivo and In Vitro Analyses

Overexpression of TG2 enhances the differentiation of ectomesenchymal stem cells into neuron‑like cells and promotes functional recovery in adult rats following spinal cord injury

Isolation and characterization of ovine umbilical cord-derived mesenchymal stem cells

Invasion of white matter tracts by glioma stem cells is regulated by a NOTCH1–SOX2 positive-feedback loop

E-Configuration Improves Antioxidant and Cytoprotective Capacities of Resveratrols

Antioxidant and Cytoprotective Effects of Kukoamines A and B: Comparison and Positional Isomeric Effect

Co-cultured the MSCs and cardiomyocytes can promote the growth of cardiomyocytes

Two phenolic antioxidants in Suoyang enhance viability of •OH-damaged mesenchymal stem cells: comparison and mechanistic chemistry

Role of the p-Coumaroyl Moiety in the Antioxidant and Cytoprotective Effects of Flavonoid Glycosides: Comparison of Astragalin and Tiliroside

Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

Lyophilized aqueous extracts of Mori Fructus and Mori Ramulus protect Mesenchymal stem cells from •OH–treated damage: bioassay and antioxidant mechanism

Synergistic effects of G-CSF and bone marrow stromal cells on nerve regeneration with acellular nerve xenografts

Sarcandra glabra (Caoshanhu) protects mesenchymal stem cells from oxidative stress: a bioevaluation and mechanistic chemistry

Mechanistic Chemistry of Extraordinary Capacity of Salvianolic Acid B on Oxidatively-damaged Mesenchymal Stem Cells

Comparison of the Antioxidant Effects of Quercitrin and Isoquercitrin: Understanding the Role of the 6″-OH Group

Protective Effects of Dihydromyricetin against •OH-Induced Mesenchymal Stem Cells Damage and Mechanistic Chemistry

Protective Effect of Sinapine against Hydroxyl Radical-Induced Damage to Mesenchymal Stem Cells and Possible Mechanisms

Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma

Overexpression of transcription factor Foxa2 and Hnf1α induced rat bone mesenchymal stem cells into hepatocytes

Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro

Induction of Functional Hepatocyte-Like Cells by Overexpression of FOXA3 and HNF4α in Rat Bone Marrow Mesenchymal Stem Cells

PPARγ and MyoD are differentially regulated by myostatin in adipose-derived stem cells and muscle satellite cells

Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism

Herba Ecliptae Protects against Hydroxyl Radical-induced Damages to DNA and Mesenchymal Stem Cells via Antioxidant Mechanism

Maclurin protects against hydroxyl radical-induced damages to mesenchymal stem cells: Antioxidant evaluation and mechanistic insight

Comparison of administration routes for adipose-derived stem cells in the treatment of middle cerebral artery occlusion in rats

Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes

Overexpression of Inducible Nitric Oxide Synthase Impairs the Survival of Bone marrow Stem Cells Transplanted into Rat Infarcted Myocardium

Endogenous release of female hormones from co-microencapsulated rat granulosa and theca cells

An effective inducer of dopaminergic neuron-like differentiation

Mineralized and osteoid tissue from dental pulp stem cells on micro-arc oxidation titanium in vitro

Therapeutic gene expression in transduced mesenchymal stem cells can be monitored using a reporter gene

Effects of total flavonoids from Drynaria fortunei on the proliferation and osteogenic differentiation of rat dental pulp stem cells

A multimodality reporter gene for monitoring transplanted stem cells

Different regulation role of myostatin in differentiating pig ADSCs and MSCs into adipocytes

Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I

Effect of Fluid Shear Stress on Cardiomyogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury

Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation

Characterization of chemical components in extracts from Si-wu decoction with proliferation-promoting effects on rat mesenchymal stem cells

Mechanical stretch promotes proliferation of rat bone marrow mesenchymal stem cells

Extracts from plastrum testudinis promote proliferation of rat bone-marrow-derived mesenchymal stem cells

Review

Have you used Anti-CD44 Antibody Picoband®?

1 Review For Anti-CD44 Antibody Picoband®

This antibody is highly efficient and specific, suitable for Western blot detection of CD44 protein in rat colon tissue, with only minor nonspecific bands.