Click image to see more details
Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
Customers Who Bought This Also Bought
Anti-GABA A Receptor beta 3/GABRB3 Antibody Picoband™
Boster Bio Anti-GABA A Receptor beta 3/GABRB3 Antibody Picoband™ catalog # PB9595. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GABA A Receptor beta 3/GABRB3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9595)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human GABRB3 (344-375aa EKTAKAKNDRSKSESNRVDAHGNILLTSLEVH), different from the related mouse and rat sequences by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB9595 is reactive to GABRB3 in Human, Mouse, Rat
PB9595 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
Submit A Review
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GABRB3 using anti-GABRB3 antibody (PB9595).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABRB3 antigen affinity purified polyclonal antibody (Catalog # PB9595) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABRB3 at approximately 54 kDa. The expected band size for GABRB3 is at 54 kDa.
Gene/Protein Information For GABRB3 (Source: Uniprot.Org, NCBI)
Gamma-aminobutyric acid receptor subunit beta-3
ligand-gated ion channel (TC 1.A.9) family
ECA5; GABA A R beta 3; GABA(A) receptor beta-3 subunit; GABA(A) receptor subunit beta-3; GABAA receptor beta-3 subunit; GABA-alpha receptor beta-2 subunit; GABAAR beta 3; GABAARb3; GABRB3; gamma-aminobutyric acid (GABA) A receptor, beta 3; gamma-aminobutyric acid receptor subunit beta-3; MGC9051 GABRB3 DEE43, ECA5, EIEE43 gamma-aminobutyric acid type A receptor subunit beta3 gamma-aminobutyric acid receptor subunit beta-3|GABA-alpha receptor beta-2 subunit|GABAA receptor beta-3 subunit|gamma-aminobutyric acid (GABA) A receptor, beta 3|gamma-aminobutyric acid A receptor beta 3|gamma-aminobutyric acid type A receptor beta3 subunit*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on GABRB3, check out the GABRB3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GABRB3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for PB9595
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Anti-GABA A Receptor beta 3/GABRB3 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-GABA A Receptor beta 3/GABRB3 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question