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Product Info Summary
| SKU: | PB9595 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GABA A Receptor beta 3/GABRB3 Antibody Picoband®
SKU/Catalog Number
PB9595
PB0627 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GABA A Receptor beta 3/GABRB3 Antibody Picoband® catalog # PB9595. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GABA A Receptor beta 3/GABRB3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9595)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GABRB3, different from the related mouse and rat sequences by five amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9595 is reactive to GABRB3 in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.1 kDa
Background of GABRB3
This gene encodes a member of the ligand-gated ionic channel family. The encoded protein is one the subunits of a multi-subunit chloride channel that serves as the receptor for gamma-aminobutyric acid, a major inhibitory neurotransmitter of the mammalian nervous system. And this gene is located on the long arm of chromosome 15 in a cluster with two other genes encoding related subunits of the family. It may be associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, nonsyndromic orofacial clefts, epilepsy and autism. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9595 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue
Validation Images & Assay Conditions
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Western blot analysis of GABRB3 using anti-GABRB3 antibody (PB9595).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABRB3 antigen affinity purified polyclonal antibody (Catalog # PB9595) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABRB3 at approximately 54 kDa. The expected band size for GABRB3 is at 54 kDa.
Specific Publications For Anti-GABA A Receptor beta 3/GABRB3 Antibody Picoband® (PB9595)
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1 Customer Q&As for Anti-GABA A Receptor beta 3/GABRB3 Antibody Picoband®
Question
We are currently using anti-GABA A Receptor beta 3/GABRB3 antibody PB9595 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
L. Mangal
Verified customer
Asked: 2017-06-07
Answer
The anti-GABA A Receptor beta 3/GABRB3 antibody (PB9595) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-07
