Anti-GALE Antibody Picoband™

GALE antibody

Boster Bio Anti-GALE Antibody Picoband™ catalog # A00551-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00551-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-GALE Antibody Picoband™

View all GALE Antibodies

SKU/Catalog Number

A00551-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GALE Antibody Picoband™ catalog # A00551-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GALE Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00551-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human GALE recombinant protein (Position: M1-N340).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00551-1 is reactive to GALE in Human, Mouse, Rat

Applications

A00551-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

38.282kDa

Background of GALE

The enzyme UDP-glucose 4-epimerase, also known as UDP-galactose 4-epimerase or GALE, is a homodimeric epimerase found in bacterial, fungal, plant, and mammalian cells. This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For GALE (Source: Uniprot.org, NCBI)

Gene Name

GALE

Full Name

UDP-glucose 4-epimerase

Weight

38.282kDa

Superfamily

NAD(P)-dependent epimerase/dehydratase family

Alternative Names

EC 5.1.3; EC 5.1.3.2; FLJ95174; FLJ97302; Galactowaldenase; short chain dehydrogenase/reductase family 1E, member 1; UDP galactose-4'-epimerase; UDP-; UDP-galactose 4-epimerase; UDP-galactose-4-epimerase; UDP-glucose 4-epimerase GALE SDR1E1 UDP-galactose-4-epimerase UDP-glucose 4-epimerase|UDP galactose-4-epimerase|UDP-GalNAc 4-epimerase|UDP-GlcNAc 4-epimerase|UDP-N-acetylgalactosamine 4-epimerase|UDP-N-acetylglucosamine 4-epimerase|epididymis secretory sperm binding protein|galactose-4-epimerase, UDP-|galactowaldenase|short chain dehydrogenase/reductase family 1E, member 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GALE, check out the GALE Infographic

GALE infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GALE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-GALE Antibody Picoband™

Question

We are currently using anti-GALE antibody A00551-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

K. Jha

Verified customer

Asked: 2019-11-05

Answer

The anti-GALE antibody (A00551-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-05

Order DetailsPrice
A00551-1

100μg

$370
A00551-1-10ug

10μg sample (liquid)

$99
A00551-1-Biotin

100 μg Biotin conjugated

$570
A00551-1-Cy3

100 μg Cy3 conjugated

$570
A00551-1-Dylight488

100 μg Dylight488 conjugated

$570
A00551-1-Dylight550

100 μg Dylight550 conjugated

$570
A00551-1-Dylight594

100 μg Dylight594 conjugated

$570
A00551-1-FITC

100 μg FITC conjugated

$570
A00551-1-HRP

100 μg HRP conjugated

$570
A00551-1-APC

100 μg APC conjugated

$670
A00551-1-PE

100 μg PE conjugated

$670
A00551-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A00551-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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