Anti-Galectin 1/LGALS1 Antibody Picoband™

Galectin-1 antibody

Boster Bio Anti-Galectin 1/LGALS1 Antibody Picoband™ catalog # PB9240-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9240-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Galectin 1/LGALS1 Antibody Picoband™

View all Galectin-1 Antibodies

SKU/Catalog Number

PB9240-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Galectin 1/LGALS1 Antibody Picoband™ catalog # PB9240-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Galectin 1/LGALS1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9240-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Galectin 1 recombinant protein (Position: A2-D135). Human Galectin 1 shares 88% and 90% amino acid (aa) sequence identity with mouse and rat Galectin 1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9240-1 is reactive to LGALS1 in Human, Mouse, Rat

Applications

PB9240-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

14.716kDa

Background of Galectin-1

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
ELISA , 0.1-0.5μg/ml, Human, -

Validation Images & Assay Conditions

Gene/Protein Information For LGALS1 (Source: Uniprot.org, NCBI)

Gene Name

LGALS1

Full Name

Galectin-1

Weight

14.716kDa

Alternative Names

14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LGALS1, check out the LGALS1 Infographic

LGALS1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LGALS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9240-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ye Z,Huang S,Zhang Y,Mei X,Zheng H,Li M,Chen J,Lu F.Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model.Front Immunol.2020 Mar 13;11:146.doi:10.3389/fimmu.2020.00146.PMID:32231658;
Species: Mouse
PB9240-1 usage in article: APP:IHC, SAMPLE:LIVER,SPLEEN AND INTESTINE TISSUE, DILUTION:1:500

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17 Customer Q&As for Anti-Galectin 1/LGALS1 Antibody Picoband™

Question

Is this PB9240-1 anti-Galectin 1/LGALS1 antibody reactive to the isotypes of LGALS1?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

The immunogen of PB9240-1 anti-Galectin 1/LGALS1 antibody is E. coli-derived human Galectin 1 recombinant protein (Position: A2-D135). Human Galectin 1 shares 88% and 90% amino acid (aa) sequence identity with mouse and rat Galectin 1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-30

Question

Will PB9240-1 anti-Galectin 1/LGALS1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

It shows on the product datasheet, PB9240-1 anti-Galectin 1/LGALS1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-20

Question

See attached the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-Galectin 1/LGALS1 antibody PB9240-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-14

Question

I was wanting to use to test anti-Galectin 1/LGALS1 antibody PB9240-1 on rat cajal-retzius cell fetal brain cortex for research purposes, then I may be interested in using anti-Galectin 1/LGALS1 antibody PB9240-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

The products we sell, including anti-Galectin 1/LGALS1 antibody PB9240-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-22

Question

Does anti-Galectin 1/LGALS1 antibody PB9240-1 work for IHC with cajal-retzius cell fetal brain cortex?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

According to the expression profile of cajal-retzius cell fetal brain cortex, LGALS1 is highly expressed in cajal-retzius cell fetal brain cortex. So, it is likely that anti-Galectin 1/LGALS1 antibody PB9240-1 will work for IHC with cajal-retzius cell fetal brain cortex.

Boster Scientific Support

Answered: 2019-11-19

Question

Our lab used your anti-Galectin 1/LGALS1 antibody for IHC on spleen in the past. I am using mouse, and We want to use the antibody for WB next. I am looking for examining spleen as well as adipose tissue in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

I looked at the website and datasheets of our anti-Galectin 1/LGALS1 antibody and I see that PB9240-1 has been tested on mouse in both IHC and WB. Thus PB9240-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-18

Question

We are currently using anti-Galectin 1/LGALS1 antibody PB9240-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

The anti-Galectin 1/LGALS1 antibody (PB9240-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-17

Question

Is a blocking peptide available for product anti-Galectin 1/LGALS1 antibody (PB9240-1)?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

We do provide the blocking peptide for product anti-Galectin 1/LGALS1 antibody (PB9240-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-03

Question

I see that the anti-Galectin 1/LGALS1 antibody PB9240-1 works with IHC, what is the protocol used to produce the result images on the product page?

R. Lewis

Verified customer

Asked: 2019-06-17

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-17

Question

Is there a BSA free version of anti-Galectin 1/LGALS1 antibody PB9240-1 available?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Galectin 1/LGALS1 antibody PB9240-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-03

Question

My colleagues were satisfied with the WB result of your anti-Galectin 1/LGALS1 antibody. However we have been able to see positive staining in adipose tissue extracellular space using this antibody. Is that expected? Could you tell me where is LGALS1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

From literature, adipose tissue does express LGALS1. Generally LGALS1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-05-16

Question

I have a question about product PB9240-1, anti-Galectin 1/LGALS1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-11-15

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9240-1 anti-Galectin 1/LGALS1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-11-15

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-Galectin 1/LGALS1 antibody PB9240-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-11-14

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-14

Question

We want using your anti-Galectin 1/LGALS1 antibody for cellular response to glucose stimulus studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-01-31

Answer

We appreciate your inquiry. This PB9240-1 anti-Galectin 1/LGALS1 antibody is tested on rat kidney, hela whole cell lysates. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-01-31

Question

I was wanting to use your anti-Galectin 1/LGALS1 antibody for IHC for rat cajal-retzius cell fetal brain cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cajal-retzius cell fetal brain cortex identification?

D. Yang

Verified customer

Asked: 2017-11-01

Answer

As indicated on the product datasheet, PB9240-1 anti-Galectin 1/LGALS1 antibody has been tested for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cajal-retzius cell fetal brain cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-01

Question

Will anti-Galectin 1/LGALS1 antibody PB9240-1 work on goat IHC with platelet?

Verified Customer

Verified customer

Asked: 2017-06-27

Answer

Our lab technicians have not validated anti-Galectin 1/LGALS1 antibody PB9240-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Galectin 1/LGALS1 antibody PB9240-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat platelet in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-27

Question

We have observed staining in rat brain. Are there any suggestions? Is anti-Galectin 1/LGALS1 antibody supposed to stain brain positively?

H. Huang

Verified customer

Asked: 2016-12-28

Answer

According to literature brain does express LGALS1. According to Uniprot.org, LGALS1 is expressed in adipose tissue, lung, placenta promyelocytic leukemia, hepatoma, placenta, lymphocyte, lung fibroblast, thalamus, placenta skin, brain, platelet, brain, cajal-retzius cell fetal brain cortex, melanoma, spleen, mammary cancer, liver, among other tissues. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-12-28

Order DetailsPrice
PB9240-1

100μg

$370
PB9240-1-10ug

10μg sample (liquid)

$99
PB9240-1-Biotin

100 μg Biotin conjugated

$570
PB9240-1-Cy3

100 μg Cy3 conjugated

$570
PB9240-1-Dylight488

100 μg Dylight488 conjugated

$570
PB9240-1-Dylight550

100 μg Dylight550 conjugated

$570
PB9240-1-Dylight594

100 μg Dylight594 conjugated

$570
PB9240-1-FITC

100 μg FITC conjugated

$570
PB9240-1-HRP

100 μg HRP conjugated

$570
PB9240-1-APC

100 μg APC conjugated

$670
PB9240-1-PE

100 μg PE conjugated

$670
PB9240-1-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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