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Product Info Summary
| SKU: | M10573 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | IP, IHC-P, ICC, WB |
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Product info
Product Name
Anti-gamma-Tubulin GCP2 Purified TUBGCP2 Monoclonal Antibody
SKU/Catalog Number
M10573
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-gamma-Tubulin GCP2 Purified TUBGCP2 Monoclonal Antibody (Catalog# M10573). Tested in IP, WB, IHC-P, ICC application(s). This antibody reacts with Rat, Mouse, Pig, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-gamma-Tubulin GCP2 Purified TUBGCP2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10573)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
GCP2-01
Isotype
Mouse IgG2b kappa
Immunogen
GST-fusion protein containing amino acids 2-194 of mouse GCP2. The mouse monoclonal antibody GCP2-01 recognizes gamma-tubulin complex component 2 (GCP2), a 95-103 kDa intracellular protein. It recognizes also phosphorylated GCP2.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M10573 is reactive to TUBGCP2 in Human, Mouse, Pig, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
102.5 kDa
Background of TUBGCP2
Gamma tubulin complex component 2 (GCP2) is 95-103 kDa protein which associates with two molecules of gamma tubulin and one molecule of gamma tubulin complex component 3 (GCP3), to form the gamma tubulin small complexes (gammaTuSCs). These complexes interact with GCP4, 5, and 6 to form the gamma tubulin ring complexes (gammaTuRCs), which are embedded in the microtubule organizing center matrix where they nucleate microtubules. The gammaTuRCs are also involved in the regulation of microtubule plus and minus end dynamics. Components of gamma tubulin complexes are highly conservative in eukaryotes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M10573 is guaranteed for IP, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1-2 μg/ml, reducing conditions.
Validation Images & Assay Conditions
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Western blotting analysis of human gamma-tubulin complex component 2 (GCP2) using mouse monoclonal antibody GCP2-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-GCP2 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GCP2 at approximately 95 kDa.
Specific Publications For Anti-gamma-Tubulin GCP2 Purified TUBGCP2 Monoclonal Antibody (M10573)
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