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- Table of Contents
Gap43 antibody (A01868-1) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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Flow Cytometry analysis of Raji cells using anti-GAP43 antibody (A01868-1).
Overlay histogram showing Raji cells stained with A01868-1 (Blue line).The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GAP43 Antibody (A01868-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Raji cells stained with A01868-1 (Blue line).The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GAP43 Antibody (A01868-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for GAP43? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the GAP43 Western Blot GuideBundle Save
Anti-GAP43 Antibody Picoband®
Gap43 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
Gap43 antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue, mouse C6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Gap43 antibody was positively detected by IHC in:
human brain tissue, rat brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Gap43 antibody was positively detected by FC in:
Raji cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Human
Tested
Recommend
Recommend
Product Information
Product Name
Anti-GAP43 Antibody Picoband®
View all GAP43 Antibodies
View all GAP43 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse GAP43 recombinant protein (Position: M1-A227).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
23.6 kDa
Background of Gap43
Growth Associated Protein 43 (GAP43) is a protein encoded by the GAP43 gene in humans. It is mapped to 3q13.31. The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Cite This Product
Anti-GAP43 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01868-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
99%
High Homology
Dog
87%
Moderate Homology
Monkey
86%
Moderate Homology
Human
85%
Moderate Homology
Cow
85%
Moderate Homology
Sheep
85%
Moderate Homology
Rabbit
83%
Moderate Homology
Zebrafish
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Gap43 antibody
Immunohistochemistry (IHC) protocol for Gap43 antibody
Flow Cytometry (FC) protocol for Gap43 antibody
Publications
Application:
Species:
Low-Dose LPS Modulates Microglia/Macrophages Phenotypic Transformation to Amplify Rehabilitation Effects in Chronic Spinal Cord Injured (CSCI) Mice
Published: 2024-02-05
Journal: MOLECULAR NEUROBIOLOGY
Application: WB
Impact Factor: 4.60
Species: Mouse
PMID: 38311654
Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury
Published: 2023-11-28
Journal: Molecular Biomedicine
Application: WB
Impact Factor: 4.00
Species: Mouse
PMID: 38015348
Adsorptive properties of graphene oxide on vitamin B12 and their effect on the promotion of peripheral nerve regeneration
Published: 2018-12-25
Journal: NEUROLOGICAL RESEARCH
Application: IF
Impact Factor: 1.45
Species: Rat
PMID: 30585138
Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Increased Cardiac Expression of Serotonin and Growth-Associated Protein-43 in Rats
Published: 2018-03-07
Journal: Biomed Research International
Application: IHC
Impact Factor: 2.58
Species: Rat
PMID: 29707580
Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system
Published: 2017-04-10
Journal: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 1.30
Species: Mouse
PMID: 28394221
Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo
Published: 2017-01-05
Journal: Scientific Reports
Application: IF
Impact Factor: 4.26
Species: Rat
PMID: 28054637
Minocycline Promotes Neurite Outgrowth of PC12 Cells Exposed to Oxygen-Glucose Deprivation and Reoxygenation Through Regulation of MLCP/MLC Signaling Pathways
Published: 2016-04-20
Journal: CELLULAR AND MOLECULAR NEUROBIOLOGY
Application: WB
Impact Factor: 2.33
Species: Rat
PMID: 27098315
Regulation of Intracellular Structural Tension by Talin in the Axon Growth and Regeneration
Published: 2015-08-23
Journal: MOLECULAR NEUROBIOLOGY
Application: ICC
Impact Factor: 5.14
Species: Mouse
PMID: 26298665
β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice
Published: 2014-01-20
Journal: BRAIN RESEARCH
Application: IHC,WB
Impact Factor: 2.83
Species: Mouse
PMID: 24457043
Volatile of alkyd varnish inhibits the expression of neuronal growth associated protein-43 in mice
Published: 2007-09-06
Journal: Neural Regeneration Research
Application: WB
Impact Factor: 0.00
Species: Mouse
Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats
Published: 2006-10-31
Journal: BRAIN RESEARCH
Application: WB
Impact Factor: 2.30
Species: Rat
PMID: 17078935
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Product Promise
We promise Anti-GAP43 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01868-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 26, 2026