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GAPDH antibody (M00227-7) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 105 Publications

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m00227 7 gapdh primary antibodies if testing 3

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Immunofluorescent analysis of Hela cells, using GAPDH Mouse Monoclonal Antibody.

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Anti-GAPDH Mouse Monoclonal Antibody

GAPDH antibody

105 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Mouse	Flow Cytometry, IP, IF, IHC, ICC, WB

Tested Applications

GAPDH antibody was positively detected by WB in:

human Hela whole cell, human Jurkat whole cell, human THP-1 whole cell, human A549 whole cell, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

GAPDH antibody was positively detected by IHC in:

human colon cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

GAPDH antibody was positively detected by ICC/IF in:

Hela cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-GAPDH Mouse Monoclonal Antibody
View all GAPDH Antibodies

Form

Liquid

Uniprot

P04406

Gene ID

2597

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Mouse

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

17G14

Isotype

IgG

Immunogen

A synthesized peptide derived from human GAPDH Mouse Monoclonal

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

36 kDa

Calculated Molecular Weight

36.1 kDa

Cite This Product

Anti-GAPDH Mouse Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-7)

Protocols

Western Blot protocol for GAPDH antibody

Review Protocol

Immunohistochemistry (IHC) protocol for GAPDH antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for GAPDH antibody

Review Protocol

Publications

Oncogenic DCTPP1/MYC feedback loop rewires pyrimidine metabolism to drive hepatocellular carcinoma

Published: 2026-05-01

Journal: FUNCTIONAL & INTEGRATIVE GENOMICS

Application: WB

Impact Factor: 4.00

Species: Human

PMID: 42062634

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Free Secondary Antibody

Anti-GAPDH Mouse Monoclonal Antibody

GAPDH antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Oncogenic DCTPP1/MYC feedback loop rewires pyrimidine metabolism to drive hepatocellular carcinoma

Attenuation of cerebral ischemia–reperfusion injury by exogenous hydrogen sulphide: involvement of the Nrf2/HO-1 pathway in NLRP3 inflammasome inhibition

Comparative Analysis of Red Onion-Derived Exosome-Like Nanovesicles and Extract Reveals Sustained Immunomodulatory Effects in LPS/IFN-γ-Stimulated Microglia

A polysaccharide from Pueraria lobata ameliorates hepatic fibrosis via gut microbiota-dependent suppression of ferroptosis

Anti-asthmatic effects and ingredient annotation of the active fraction from Ganoderma lucidum

TNF-α/PAD4 axis drives hyperhomocysteine-induced white matter injury after acute ischemic stroke

A novel polysaccharide from Polygonatum cyrtonema fibrous root mediates anti-diarrheal effects by reprogramming macrophage M1/M2 polarization

Identification of intracellular target of antimicrobial peptide EWAMP-R in bacteria

Integrated Network Analysis Reveals a Directly Regulatory Network of FOXM1 Associated With the Cell Cycle in Lung Adenocarcinoma

Sulfasalazine induces ferroptosis in osteosarcomas by regulating Nrf2/SLC7A11/GPX4 signaling axis

PAI-1 regulates extracellular matrix remodeling and alters fibroblast profibrotic ability in skeletal muscle repair

TRUB1 is a novel biomarker for promoting malignancy in colorectal cancer via NFκB signaling

Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity

Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study

Identifying Hepsin as a novel biomarker for human esophageal squamous cell carcinoma (ESCC) and its application in fluorescence imaging

Changes in early endosomes in rat hippocampal CA1 neurons after transient global cerebral ischaemia

The PGC-1α/ERRα/ULK1 pathway contributes to Perioperative neurocognitive disorders by inducing mitochondrial dysfunction and activating NLRP3 inflammasome in aged mice

Pulmonary artery smooth muscle cell pyroptosis promotes the proliferation of PASMCs by paracrine IL‑1β and IL‑18 in monocrotaline‑induced pulmonary arterial hypertensive rats

Mechanism underlying the role of integrin α3β1 in adhesive dysfunction between thyroid cells induced by diesel engine exhaust particles

Loss of SIL1 Affects Actin Dynamics and Leads to Abnormal Neural Migration

Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer

Enhanced therapeutic potential of Flotillins-modified MenSCs by improve the survival, proliferation and migration

IFIH1-mediated post-transcriptional regulation of PTTG1 promotes proliferation and affects PHA-848125 sensitivity and prognosis in oropharyngeal carcinoma

Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability

Tamarix aphylla derived metabolites ameliorate indomethacin-induced gastric ulcers in rats by modulating the MAPK signaling pathway, alleviating oxidative stress and inflammation: In vivo study supported by pharmacological network analysis

Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study

Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway

T-2 toxin induces cardiac fibrosis by causing metabolic disorders and up-regulating Sirt3/FoxO3α/MnSOD signaling pathway-mediated oxidative stress

Exogenous hydrogen sulfide ameliorates diabetes-associated cognitive dysfunction by regulating the nrf-2/HO-1 axis and the NLRP3 inflammasome pathway in diabetic rats

Copper metabolism-related risk score identifies hepatocellular carcinoma subtypes and SLC27A5 as a potential regulator of cuproptosis

Low ambient temperature exposure increases the risk of ischemic stroke by promoting platelet activation

METTL3 and METTL14 regulate IL-6 expression via RNA m6A modification of zinc transporter SLC39A9 and DNA methylation of IL-6 in periodontal ligament cells

Constitutive aryl hydrocarbon receptor facilitates the regenerative potential of mouse bone marrow mesenchymal stromal cells

Role and mechanism of Circ-PDE7B in the formation of keloid

Inhibition of TRADD ameliorates chondrocyte necroptosis and osteoarthritis by blocking RIPK1-TAK1 pathway and restoring autophagy

Role of N6-adenosine-methyltransferase subunits METTL3 and METTL14 in the biological properties of periodontal ligament cells

Prenatal LPS Exposure Promotes Allergic Airway Inflammation via Long Coding RNA NONMMUT033452.2, and Protein Binding Partner, Eef1D

RETRACTED ARTICLE: MiR-935 Promotes Clear Cell Renal Cell Carcinoma Migration and Invasion by Targeting IREB2

Variation in pH, HCO3−, carbonic anhydrases, and HCO3− transporters in Nile tilapia during carbonate alkalinity stress

Recruitment of LEF1 by Pontin chromatin modifier amplifies TGFBR2 transcription and activates TGFβ/SMAD signalling during gliomagenesis

Decoy receptor 2 mediates the apoptosis-resistant phenotype of senescent renal tubular cells and accelerates renal fibrosis in diabetic nephropathy

The JMJD3 histone demethylase inhibitor GSK-J1 ameliorates lipopolysaccharide-induced inflammation in a mastitis model

Terminalia chebula extracts ameliorate methamphetamine-induced memory deficits via activating the ERK and Nrf2 pathway

Differently Charged P (VDF-TrFE) Membranes Influence Osteogenesis Through Differential Immunomodulatory Function of Macrophages

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats

Influence of miRNA-30a-5p on Pulmonary Fibrosis in Mice with Streptococcus pneumoniae Infection through Regulation of Autophagy by Beclin-1

Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway

Tripartite motif-containing 3 (TRIM3) enhances ER signaling and confers tamoxifen resistance in breast cancer

A novel method to obtain rat aortic media for primary culture of rat aortic smooth muscle cells

Lycopene Alleviates Titanium Dioxide Nanoparticle-Induced Testicular Toxicity by Inhibiting Oxidative Stress and Apoptosis in Mice

An investigation on nephrotoxicity of Aristolactam I induced epithelial-mesenchymal transition on HK-2 cells

ILK regulates osteogenic differentiation of Human Periodontal Ligament Stem Cells through YAP-mediated Mechanical Memory

Fab Fragment of Immunoglobulin Y Modulates NF-κB and MAPK Signaling through TLR4 and αVβ3 Integrin and Inhibits the Inflammatory Effect on R264.7 Macrophages

NRF‑1 directly regulates TFE3 and promotes the proliferation of renal cancer cells

Changes in Expressions of HSP27, HSP70, and Soluble Glycoprotein in Heart Failure Rats Complicated with Pulmonary Edema and Correlations with Cardiopulmonary Functions

A Preliminary Study on the Promotion of Canine Adipose-Derived Stem Cell Differentiation by Perfusion-Decellularized Ureter Matrix

12-Epi-Napelline Inhibits Leukemia Cell Proliferation via the PI3K/AKT Signaling Pathway In Vitro and In Vivo

Chondroprotective and antiarthritic effects of galangin in osteoarthritis: An in vitro and in vivo study

Rhoifolin Ameliorates Osteoarthritis via Regulating Autophagy

Resveratrol attenuates methamphetamine-induced memory impairment via inhibition of oxidative stress and apoptosis in mice

Identification of HIPK3 as a potential biomarker and an inhibitor of clear cell renal cell carcinoma

Cancer/testis antigen LDHC promotes proliferation and metastasis by activating the PI3K/Akt/GSK-3β-signaling pathway and the in lung adenocarcinoma

Non-muscle myosin heavy chain 9 is a critical factor for infectious pancreatic necrosis virus cellular entry

Sodium Butyrate Alleviates Lipopolysaccharide-Induced Inflammatory Responses by Down-Regulation of NF-κB, NLRP3 Signaling Pathway, and Activating Histone Acetylation in Bovine Macrophages

Hyperoside ameliorates the progression of osteoarthritis: An in vitro and in vivo study

PIK3R3 inhibits cell senescence through p53/p21 signaling

Neuroprotective Effects of Celastrol on Transient Global Cerebral Ischemia Rats via Regulating HMGB1/NF-κB Signaling Pathway