Anti-GAPDH Rabbit Monoclonal Antibody

Product Name

Anti-GAPDH Rabbit Monoclonal Antibody

View all GAPDH Antibodies

SKU/Catalog Number

M00227

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody catalog # M00227. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAPDH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BH-7

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human GAPDH

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00227 is reactive to GAPDH in Human, Mouse, Rat

Applications

M00227 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

58 kDa

Calculated molecular weight

36.053kDa

Background of GAPDH

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:50000
IHC 1:100-1:500
ICC/IF 1:100-1:250
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Antibody.

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All lanes use GAPDH Antibody at 1:50000 dilution for 1 hour at room temperature.

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All lanes use GAPDH Antibody at 1:50000 dilution for 1 hour at room temperature.

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Immunohistochemical analysis of paraffin-embedded (1) Rat colon; (2) Mouse testis; (3) Human uterus; (4) Human kidney, using GAPDH Antibody .

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Immunohistochemical analysis of paraffin-embedded Human pituitary adenoma, using the Antibody at 1:400 dilution.

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Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:400 dilution.

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Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:400 dilution.

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Immunofluorescent analysis of Hela cells, using GAPDH Antibody .

Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)

Alternative Names

1D4 GAPDH; aging-associated gene 9 protein; Cytoplasm Marker; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PD; G3PDH; GAPD; GAPDH; GAPDPeptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38; OCT1 coactivator in S phase; Peptidyl-cysteine S-nitrosylase GAPDH GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH

For more info on GAPDH, check out the GAPDH Infographic

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In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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