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Product Info Summary
| SKU: | A00608-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-GAS 6/GAS6 Antibody Picoband®
SKU/Catalog Number
A00608-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GAS 6/GAS6 Antibody Picoband® catalog # A00608-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAS 6/GAS6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00608-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GAS 6 recombinant protein (Position: M488-S660). Human GAS 6 shares 79.7% and 81.4% amino acid (aa) sequence identity with mouse and rat GAS 6, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00608-1 is reactive to GAS6 in Human, Mouse, Rat
Observed Molecular Weight
55, 79 kDa
Calculated molecular weight
74.9 kDa
Background of GAS6
Growth arrest-specific 6, also known as GAS6, is a human gene coding for the Gas6 protein. It is similar to the Protein S with the same domain organization and 43% amino acid identity. It was originally found as a gene upregulated by growth arrested fibroblasts. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00608-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human THP-1 whole cell, human A431 whole cell, rat brain tissue, mouse brain tissue
IHC: human lung cancer tissue
FCM: THP-1 cell
Validation Images & Assay Conditions
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Western blot analysis of GAS6 using anti-GAS6 antibody (A00608-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS6 antigen affinity purified polyclonal antibody (A00608-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GAS6 at approximately 55, 79 kDa. The expected band size for GAS6 is at 79 kDa.
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IHC analysis of GAS6 using anti-GAS6 antibody (A00608-1).
GAS6 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GAS6 Antibody (A00608-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Flow Cytometry analysis of THP-1 cells using anti-GAS6 antibody (A00608-1).
Overlay histogram showing THP-1 cells stained with A00608-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GAS6 Antibody (A00608-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-GAS 6/GAS6 Antibody Picoband® (A00608-1)
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Customer Q&As
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17 Customer Q&As for Anti-GAS 6/GAS6 Antibody Picoband®
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thoracic aorta using anti-GAS 6/GAS6 antibody A00608-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-30
Question
Is this A00608-1 anti-GAS 6/GAS6 antibody reactive to the isotypes of GAS6?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The immunogen of A00608-1 anti-GAS 6/GAS6 antibody is E.coli-derived human GAS 6 recombinant protein (Position: M488-S660). Human GAS 6 shares 79.7% and 81.4% amino acid (aa) sequence identity with mouse and rat GAS 6, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-27
Question
We are currently using anti-GAS 6/GAS6 antibody A00608-1 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The anti-GAS 6/GAS6 antibody (A00608-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-30
Question
Our lab used your anti-GAS 6/GAS6 antibody for WB on cervix carcinoma a few months ago. I am using mouse, and I plan to use the antibody for IHC next. I would like examining cervix carcinoma as well as fetal lung fetal spleen in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
I have checked the website and datasheets of our anti-GAS 6/GAS6 antibody and I see that A00608-1 has been validated on mouse in both WB and IHC. Thus A00608-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-17
Question
I am interested in to test anti-GAS 6/GAS6 antibody A00608-1 on mouse thoracic aorta for research purposes, then I may be interested in using anti-GAS 6/GAS6 antibody A00608-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
The products we sell, including anti-GAS 6/GAS6 antibody A00608-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-12
Question
Is there a BSA free version of anti-GAS 6/GAS6 antibody A00608-1 available?
Verified Customer
Verified customer
Asked: 2019-03-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAS 6/GAS6 antibody A00608-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-08
Question
Is a blocking peptide available for product anti-GAS 6/GAS6 antibody (A00608-1)?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
We do provide the blocking peptide for product anti-GAS 6/GAS6 antibody (A00608-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-02-08
Question
I see that the anti-GAS 6/GAS6 antibody A00608-1 works with WB, what is the protocol used to produce the result images on the product page?
C. Zhang
Verified customer
Asked: 2018-11-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-11-13
Question
We have been able to see staining in human thoracic aorta. Are there any suggestions? Is anti-GAS 6/GAS6 antibody supposed to stain thoracic aorta positively?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
According to literature thoracic aorta does express GAS6. According to Uniprot.org, GAS6 is expressed in thoracic aorta, cervix carcinoma, kidney uterus, fetal lung fetal spleen, among other tissues. Regarding which tissues have GAS6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 8336730
Fetal lung, and Fetal spleen, Pubmed ID: 15489334
Kidney, and Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-05-24
Question
Would anti-GAS 6/GAS6 antibody A00608-1 work on feline WB with fetal lung fetal spleen?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
Our lab technicians have not validated anti-GAS 6/GAS6 antibody A00608-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-GAS 6/GAS6 antibody A00608-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline fetal lung fetal spleen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-20
Question
We are interested in using your anti-GAS 6/GAS6 antibody for positive regulation of cytokine-mediated signaling pathway studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
We appreciate your inquiry. This A00608-1 anti-GAS 6/GAS6 antibody is validated on rat brain tissue, mouse brain. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-07-17
Question
Does anti-GAS 6/GAS6 antibody A00608-1 work for WB with thoracic aorta?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
According to the expression profile of thoracic aorta, GAS6 is highly expressed in thoracic aorta. So, it is likely that anti-GAS 6/GAS6 antibody A00608-1 will work for WB with thoracic aorta.
Boster Scientific Support
Answered: 2017-05-24
Question
Would A00608-1 anti-GAS 6/GAS6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Li
Verified customer
Asked: 2016-11-17
Answer
As indicated on the product datasheet, A00608-1 anti-GAS 6/GAS6 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-11-17
Question
My boss were well pleased with the WB result of your anti-GAS 6/GAS6 antibody. However we have observed positive staining in thoracic aorta secreted. using this antibody. Is that expected? Could you tell me where is GAS6 supposed to be expressed?
M. Rodriguez
Verified customer
Asked: 2014-11-03
Answer
From literature, thoracic aorta does express GAS6. Generally GAS6 expresses in secreted. Regarding which tissues have GAS6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 8336730
Fetal lung, and Fetal spleen, Pubmed ID: 15489334
Kidney, and Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-11-03
Question
I have attached the WB image, lot number and protocol we used for thoracic aorta using anti-GAS 6/GAS6 antibody A00608-1. Please let me know if you require anything else.
E. Yang
Verified customer
Asked: 2013-11-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-27
Question
I was wanting to use your anti-GAS 6/GAS6 antibody for WB for mouse thoracic aorta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse thoracic aorta identification?
J. Parker
Verified customer
Asked: 2013-06-25
Answer
As indicated on the product datasheet, A00608-1 anti-GAS 6/GAS6 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse thoracic aorta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-06-25
Question
My question regarding product A00608-1, anti-GAS 6/GAS6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Li
Verified customer
Asked: 2013-06-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00608-1 anti-GAS 6/GAS6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-06-21
