Anti-GAS 6/GAS6 Antibody Picoband™

Boster Bio Anti-GAS 6/GAS6 Antibody Picoband™ catalog # A00608-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00608-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-GAS 6/GAS6 Antibody Picoband™

See all GAS6 products

SKU/Catalog Number

A00608-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GAS 6/GAS6 Antibody Picoband™ catalog # A00608-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAS 6/GAS6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00608-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human GAS 6 recombinant protein (Position: M488-S660). Human GAS 6 shares 79.7% and 81.4% amino acid (aa) sequence identity with mouse and rat GAS 6, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00608-1 is reactive to GAS6 in Human, Mouse, Rat

Applications

A00608-1 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For GAS6 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q14393

Gene ID

2621

Gene Name

GAS6

Full Name

Growth arrest-specific protein 6

Weight

74.925kDa

Alternative Names

AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6 GAS6 AXLLG, AXSF growth arrest specific 6 growth arrest-specific protein 6|AXL receptor tyrosine kinase ligand|AXL stimulatory factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GAS6, check out the GAS6 Infographic

GAS6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GAS6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00608-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chun Wang,Bin Wang,Maimaiti Aili,Shixia Huo,Meng-ting Han,Silafu Aibai,Zhi-Jian Li, "Effect of Artemisia rupestris L. Extract on Gastrointestinal Hormones and Brain-Gut Peptides in Functional Dyspepsia Rats", Evidence-Based Complementary and Alternative M
Species: Mouse
A00608-1 usage in article: APP:IHC, SAMPLE:ANTRUM AND HYPOTHALAMUS, DILUTION:NA

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Find answers in Q&As, reviews.

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17 Customer Q&As for Anti-GAS 6/GAS6 Antibody Picoband™

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thoracic aorta using anti-GAS 6/GAS6 antibody A00608-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-30

Question

Is this A00608-1 anti-GAS 6/GAS6 antibody reactive to the isotypes of GAS6?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

The immunogen of A00608-1 anti-GAS 6/GAS6 antibody is E.coli-derived human GAS 6 recombinant protein (Position: M488-S660). Human GAS 6 shares 79.7% and 81.4% amino acid (aa) sequence identity with mouse and rat GAS 6, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-27

Question

We are currently using anti-GAS 6/GAS6 antibody A00608-1 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

The anti-GAS 6/GAS6 antibody (A00608-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-30

Question

Our lab used your anti-GAS 6/GAS6 antibody for WB on cervix carcinoma a few months ago. I am using mouse, and I plan to use the antibody for IHC next. I would like examining cervix carcinoma as well as fetal lung fetal spleen in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

I have checked the website and datasheets of our anti-GAS 6/GAS6 antibody and I see that A00608-1 has been validated on mouse in both WB and IHC. Thus A00608-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-17

Question

I am interested in to test anti-GAS 6/GAS6 antibody A00608-1 on mouse thoracic aorta for research purposes, then I may be interested in using anti-GAS 6/GAS6 antibody A00608-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

The products we sell, including anti-GAS 6/GAS6 antibody A00608-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-12

Question

Is there a BSA free version of anti-GAS 6/GAS6 antibody A00608-1 available?

Verified Customer

Verified customer

Asked: 2019-03-08

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAS 6/GAS6 antibody A00608-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-03-08

Question

Is a blocking peptide available for product anti-GAS 6/GAS6 antibody (A00608-1)?

Verified Customer

Verified customer

Asked: 2019-02-08

Answer

We do provide the blocking peptide for product anti-GAS 6/GAS6 antibody (A00608-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-02-08

Question

I see that the anti-GAS 6/GAS6 antibody A00608-1 works with WB, what is the protocol used to produce the result images on the product page?

C. Zhang

Verified customer

Asked: 2018-11-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-11-13

Question

We have been able to see staining in human thoracic aorta. Are there any suggestions? Is anti-GAS 6/GAS6 antibody supposed to stain thoracic aorta positively?

Verified Customer

Verified customer

Asked: 2018-05-24

Answer

According to literature thoracic aorta does express GAS6. According to Uniprot.org, GAS6 is expressed in thoracic aorta, cervix carcinoma, kidney uterus, fetal lung fetal spleen, among other tissues. Regarding which tissues have GAS6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 8336730
Fetal lung, and Fetal spleen, Pubmed ID: 15489334
Kidney, and Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-05-24

Question

Would anti-GAS 6/GAS6 antibody A00608-1 work on feline WB with fetal lung fetal spleen?

Verified Customer

Verified customer

Asked: 2017-07-20

Answer

Our lab technicians have not validated anti-GAS 6/GAS6 antibody A00608-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-GAS 6/GAS6 antibody A00608-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline fetal lung fetal spleen in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-20

Question

We are interested in using your anti-GAS 6/GAS6 antibody for positive regulation of cytokine-mediated signaling pathway studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-07-17

Answer

We appreciate your inquiry. This A00608-1 anti-GAS 6/GAS6 antibody is validated on rat brain tissue, mouse brain. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-07-17

Question

Does anti-GAS 6/GAS6 antibody A00608-1 work for WB with thoracic aorta?

Verified Customer

Verified customer

Asked: 2017-05-24

Answer

According to the expression profile of thoracic aorta, GAS6 is highly expressed in thoracic aorta. So, it is likely that anti-GAS 6/GAS6 antibody A00608-1 will work for WB with thoracic aorta.

Boster Scientific Support

Answered: 2017-05-24

Question

Would A00608-1 anti-GAS 6/GAS6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Li

Verified customer

Asked: 2016-11-17

Answer

As indicated on the product datasheet, A00608-1 anti-GAS 6/GAS6 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-11-17

Question

My boss were well pleased with the WB result of your anti-GAS 6/GAS6 antibody. However we have observed positive staining in thoracic aorta secreted. using this antibody. Is that expected? Could you tell me where is GAS6 supposed to be expressed?

M. Rodriguez

Verified customer

Asked: 2014-11-03

Answer

From literature, thoracic aorta does express GAS6. Generally GAS6 expresses in secreted. Regarding which tissues have GAS6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 8336730
Fetal lung, and Fetal spleen, Pubmed ID: 15489334
Kidney, and Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2014-11-03

Question

I have attached the WB image, lot number and protocol we used for thoracic aorta using anti-GAS 6/GAS6 antibody A00608-1. Please let me know if you require anything else.

E. Yang

Verified customer

Asked: 2013-11-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-11-27

Question

I was wanting to use your anti-GAS 6/GAS6 antibody for WB for mouse thoracic aorta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse thoracic aorta identification?

J. Parker

Verified customer

Asked: 2013-06-25

Answer

As indicated on the product datasheet, A00608-1 anti-GAS 6/GAS6 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse thoracic aorta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-06-25

Question

My question regarding product A00608-1, anti-GAS 6/GAS6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Li

Verified customer

Asked: 2013-06-21

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00608-1 anti-GAS 6/GAS6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-06-21

Conjugation

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A00608-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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