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Product Info Summary
| SKU: | A13756 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GAS PAGR1 Antibody
SKU/Catalog Number
A13756
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GAS PAGR1 Antibody (Catalog # A13756). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
GAS antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-GAS PAGR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13756)
Host
Rabbit
Contents
GAS Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GAS antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human GAS. The immunogen is located within the last 50 amino acids of GAS.
Reactive Species
A13756 is reactive to PAGR1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.7 kDa
Background of PAGR1
Steroid receptor co-activators (SRCs) were initially described as nuclear receptor transcription co-activators, but they have recently been determined to co-regulate transcription initiated by other transcription factors. GAS is a recently identified glutamate-rich protein that interacts with SRC1, but not GRIP1 or AIB1, the other two members of the SRC family. GAS can also interact with the alpha subunit of the estrogen receptor (ERalpha), but not other receptors such as the retinoic acid receptor a, suggesting the interaction between GAS and ERalpha is relatively specific. Depletion of GAS by RNA interference in MCF7 cells led to a decrease in the mRNA and protein levels of ER target genes such as pS2, c-Myc and cyclin D1, indicating the role of GAS in the regulation of ER target genes. GAS has also been found to associate with an SET1-like methyltransferase complex specific for H3K4 methylation, suggesting that GAS has multiple roles in transcriptional regulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13756 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GAS antibody can be used for detection of GAS by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GAS in EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide with GAS antibody at 1μg/mL.
Specific Publications For Anti-GAS PAGR1 Antibody (A13756)
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